An Easy and Efficient Procedure for the Isolation of Pure DNA Restriction Fragments from Agarose Gels

A.M. Ledeboer, J. Hille, R.A. Schilperoort

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Abstract

A new procedure is developed to isolate DNA from agarose gels. Using a kind of blotting technique, DNA is isolated from the gel. It is shown that the isolated DNA can be used for fragmentation by restriction endonucleases, synthesis of complementary RNA by DNA-dependent RNA polymerase from Escherichia coli and nick translation. The procedure gives a high recovery and is easy to perform.
Original languageEnglish
Number of pages7
JournalBiochimica et Biophysica Acta %28BBA%29 - Nucleic Acids and Protein Synthesis
Volume520
Issue number3
DOIs
Publication statusPublished - 1978

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