An Easy and Efficient Procedure for the Isolation of Pure DNA Restriction Fragments from Agarose Gels

A.M. Ledeboer; J. Hille; R.A. Schilperoort

doi:10.1016/0005-2787(78)90135-1

An Easy and Efficient Procedure for the Isolation of Pure DNA Restriction Fragments from Agarose Gels

A.M. Ledeboer, J. Hille, R.A. Schilperoort

Groningen Biomolecular Sciences and Biotechnology Institute

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Abstract

A new procedure is developed to isolate DNA from agarose gels. Using a kind of blotting technique, DNA is isolated from the gel. It is shown that the isolated DNA can be used for fragmentation by restriction endonucleases, synthesis of complementary RNA by DNA-dependent RNA polymerase from Escherichia coli and nick translation. The procedure gives a high recovery and is easy to perform.

Original language	English
Number of pages	7
Journal	Biochimica et Biophysica Acta %28BBA%29 - Nucleic Acids and Protein Synthesis
Volume	520
Issue number	3
DOIs	https://doi.org/10.1016/0005-2787(78)90135-1
Publication status	Published - 1978

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Cite this

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title = "An Easy and Efficient Procedure for the Isolation of Pure DNA Restriction Fragments from Agarose Gels",

abstract = "A new procedure is developed to isolate DNA from agarose gels. Using a kind of blotting technique, DNA is isolated from the gel. It is shown that the isolated DNA can be used for fragmentation by restriction endonucleases, synthesis of complementary RNA by DNA-dependent RNA polymerase from Escherichia coli and nick translation. The procedure gives a high recovery and is easy to perform.",

author = "A.M. Ledeboer and J. Hille and R.A. Schilperoort",

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AU - Ledeboer, A.M.

AU - Hille, J.

AU - Schilperoort, R.A.

N1 - Relation: http://www.rug.nl/gbb/ date_submitted:2007 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute

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N2 - A new procedure is developed to isolate DNA from agarose gels. Using a kind of blotting technique, DNA is isolated from the gel. It is shown that the isolated DNA can be used for fragmentation by restriction endonucleases, synthesis of complementary RNA by DNA-dependent RNA polymerase from Escherichia coli and nick translation. The procedure gives a high recovery and is easy to perform.

AB - A new procedure is developed to isolate DNA from agarose gels. Using a kind of blotting technique, DNA is isolated from the gel. It is shown that the isolated DNA can be used for fragmentation by restriction endonucleases, synthesis of complementary RNA by DNA-dependent RNA polymerase from Escherichia coli and nick translation. The procedure gives a high recovery and is easy to perform.

U2 - 10.1016/0005-2787(78)90135-1

DO - 10.1016/0005-2787(78)90135-1

M3 - Article

VL - 520

JO - Biochimica et Biophysica Acta %28BBA%29 - Nucleic Acids and Protein Synthesis

JF - Biochimica et Biophysica Acta %28BBA%29 - Nucleic Acids and Protein Synthesis

SN - 1878-2434

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ER -