AN IMAGE-ANALYSIS TECHNIQUE FOR DETECTION OF RADIATION-INDUCED DNA FRAGMENTATION AFTER CHEF ELECTROPHORESIS

M ROSEMANN, B KANON, AWT KONINGS, HH KAMPINGA

    Research output: Contribution to journalComment/Letter to the editorAcademicpeer-review

    25 Citations (Scopus)

    Abstract

    CHEF-electrophoresis was used as a technique to detect radiation-induced DNA breakage with special emphasis to biological relevant X-ray doses (0-10 Gy). Fluorescence detection of DNA-fragments using a sensitive image analysis system was directly compared with conventional scintillation counting of H-3-thymidine prelabelled DNA in HeLa S3 cells. It is shown that the image analysis-based fluorescence detection of fragmented DNA after ionizing radiation is as sensitive and reproducible as detection using radioactively prelabelled cells without the putative shortcomings of fluorescence detection methods described earlier (Blocher and Kuhni 1990). Therefore, the image analysis-based detection of radiation-induced DNA fragmentation after CHEF electrophoresis seems to be the most reliable method for applications to non-cycling cells and biopsy material.

    Original languageEnglish
    Pages (from-to)245-249
    Number of pages5
    JournalInternational Journal of Radiation Biology
    Volume64
    Issue number2
    Publication statusPublished - Aug-1993

    Keywords

    • DOUBLE-STRAND BREAKS
    • MAMMALIAN-CELLS

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