An Optical Probe for Real-Time Monitoring of Self-Replicator Emergence and Distinguishing between Replicators

Joydev Hatai, Yigit Altay, Ankush Sood, Armin Kiani, Marcel J. Eleveld, Leila Motiei, David Margulies*, Sijbren Otto*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)
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Self-replicating systems play an important role in research on the synthesis and origin of life. Monitoring of these systems has mostly relied on techniques such as NMR or chromatography, which are limited in throughput and demanding when monitoring replication in real time. To circumvent these problems, we now developed a pattern-generating fluorescent molecular probe (an ID-probe) capable of discriminating replicators of different chemical composition and monitoring the process of replicator formation in real time, giving distinct signatures for starting materials, intermediates, and final products. Optical monitoring of replicators dramatically reduces the analysis time and sample quantities compared to most currently used methods and opens the door for future high-throughput experimentation in protocell environments.

Original languageEnglish
Pages (from-to)3074-3082
Number of pages9
JournalJournal of the American Chemical Society
Issue number7
Publication statusPublished - 23-Feb-2022

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