An Overview of the Top Ten Detergents Used for Membrane Protein Crystallization

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To study integral membrane proteins, one has to extract them from the membrane—the step that is typically achieved by the application of detergents. In this mini-review, we summarize the top 10 detergents used for the structural analysis of membrane proteins based on the published results. The aim of this study is to provide the reader with an overview of the main properties of available detergents (critical micelle concentration (CMC) value, micelle size, etc.) and provide an idea of what detergents to may merit further study. Furthermore, we briefly discuss alternative solubilization and stabilization agents, such as polymers.
Original languageEnglish
Article number197
Pages (from-to)1-16
Number of pages16
Issue number7
Publication statusPublished - 1-Jul-2017


  • detergent
  • membrane proteins
  • SMA
  • amphipols

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