An Overview of the Top Ten Detergents Used for Membrane Protein Crystallization

Artem Stetsenko; Albert Guskov

doi:10.3390/cryst7070197

An Overview of the Top Ten Detergents Used for Membrane Protein Crystallization

Artem Stetsenko, Albert Guskov

Enzymology

Research output: Contribution to journal › Article › Academic › peer-review

95 Citations (Scopus)

13380 Downloads (Pure)

Abstract

To study integral membrane proteins, one has to extract them from the membrane—the step that is typically achieved by the application of detergents. In this mini-review, we summarize the top 10 detergents used for the structural analysis of membrane proteins based on the published results. The aim of this study is to provide the reader with an overview of the main properties of available detergents (critical micelle concentration (CMC) value, micelle size, etc.) and provide an idea of what detergents to may merit further study. Furthermore, we briefly discuss alternative solubilization and stabilization agents, such as polymers.

Original language	English
Article number	197
Pages (from-to)	1-16
Number of pages	16
Journal	Crystals
Volume	7
Issue number	7
DOIs	https://doi.org/10.3390/cryst7070197
Publication status	Published - 1-Jul-2017

Keywords

detergent
membrane proteins
SMA
amphipols

Access to Document

10.3390/cryst7070197Licence: CC BY

An Overview of the Top Ten Detergents Used for Membrane Protein CrystallizationAn Overview of the Top Ten Detergents Used for Membrane Protein CrystallizationFinal publisher's version, 4.9 MBLicence: CC BY

Handle.net

Persistent link

Cite this

@article{4bccffc920254ec6bd89c4cc6517f26b,

title = "An Overview of the Top Ten Detergents Used for Membrane Protein Crystallization",

abstract = "To study integral membrane proteins, one has to extract them from the membrane—the step that is typically achieved by the application of detergents. In this mini-review, we summarize the top 10 detergents used for the structural analysis of membrane proteins based on the published results. The aim of this study is to provide the reader with an overview of the main properties of available detergents (critical micelle concentration (CMC) value, micelle size, etc.) and provide an idea of what detergents to may merit further study. Furthermore, we briefly discuss alternative solubilization and stabilization agents, such as polymers.",

keywords = "detergent, membrane proteins, SMA, amphipols",

author = "Artem Stetsenko and Albert Guskov",

note = "This article belongs to the Special Issue Recent Advances in Protein Crystallography",

year = "2017",

month = jul,

day = "1",

doi = "10.3390/cryst7070197",

language = "English",

volume = "7",

pages = "1--16",

journal = "Crystals",

issn = "2073-4352",

publisher = "MDPI AG",

number = "7",

}

TY - JOUR

T1 - An Overview of the Top Ten Detergents Used for Membrane Protein Crystallization

AU - Stetsenko, Artem

AU - Guskov, Albert

N1 - This article belongs to the Special Issue Recent Advances in Protein Crystallography

PY - 2017/7/1

Y1 - 2017/7/1

N2 - To study integral membrane proteins, one has to extract them from the membrane—the step that is typically achieved by the application of detergents. In this mini-review, we summarize the top 10 detergents used for the structural analysis of membrane proteins based on the published results. The aim of this study is to provide the reader with an overview of the main properties of available detergents (critical micelle concentration (CMC) value, micelle size, etc.) and provide an idea of what detergents to may merit further study. Furthermore, we briefly discuss alternative solubilization and stabilization agents, such as polymers.

AB - To study integral membrane proteins, one has to extract them from the membrane—the step that is typically achieved by the application of detergents. In this mini-review, we summarize the top 10 detergents used for the structural analysis of membrane proteins based on the published results. The aim of this study is to provide the reader with an overview of the main properties of available detergents (critical micelle concentration (CMC) value, micelle size, etc.) and provide an idea of what detergents to may merit further study. Furthermore, we briefly discuss alternative solubilization and stabilization agents, such as polymers.

KW - detergent

KW - membrane proteins

KW - SMA

KW - amphipols

U2 - 10.3390/cryst7070197

DO - 10.3390/cryst7070197

M3 - Article

SN - 2073-4352

VL - 7

SP - 1

EP - 16

JO - Crystals

JF - Crystals

IS - 7

M1 - 197

ER -

An Overview of the Top Ten Detergents Used for Membrane Protein Crystallization

Abstract

Keywords

Access to Document

Handle.net

Fingerprint

Cite this