An Xp22.1-p22.2 YAC contig encompassing the disease loci for RS, KFSD, CLS, HYP and RP15: Refined localization of RS

E van de Vosse, AAB Bergen, EJ Meershoek, JC Oosterwijk, S Gregory, B Bakker, J Weissenbach, AJ Coffey, GJB vanOmmen, JT DenDunnen

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Abstract

To facilitate the positional cloning of the genes involved in retinoschisis (RS), keratosis follicularis spinulosa decalvans (KFSD), Coffin-Lowry syndrome (CLS), X-linked hypophosphatemic rickets (XLH, locus name HYP) and X-linked dominant cone-rod degeneration (locus name RP 15), we have extended the molecular map of the Xp22 region. Screening of several YAC libraries allowed us to identify 156 YACs, 52 of which localize between markers DXS414 (P90) and DXS451 (kQST80H1). Analysis of their marker content facilitated the construction of a YAC contig from the region spanning (in this order): DXS414 - DXS987 - DXS207 - DXS1053 - DXS197 - DXS43 - DXS1195 - DXS418 - DXS999 - PDHA1 - DXS7161 - DXS443 - DXS7592 - DXS1229 - DXS365 - DXS7101 - DXS7593 - DXS1052 - DXS274 - DXS989 - DXS451. The region between DXS414 and DXS451 covers about 4.5-5 Mb. Two additional markers (DXS7593 and DXS7592) were placed in the region, thereby increasing the genetic resolution. Using the deduced marker order, the analysis of key recombinants in families segregating RS allowed us to refine the critical region for RS to 0.6 Mb, between DXS418 and DXS7161.

Original languageEnglish
Pages (from-to)101-104
Number of pages4
JournalEuropean Journal of Human Genetics
Volume4
Issue number2
Publication statusPublished - 1996

Keywords

  • retinoschisis
  • Xp22
  • Contig
  • YEAST ARTIFICIAL CHROMOSOMES
  • HUMAN GENOME
  • CONSTRUCTION
  • LIBRARY

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