Analysis of 1135 gut metagenomes identifies sex-specific resistome profiles

Trishla Sinha; Arnau Vich Vila; Sanzhima Garmaeva; Soesma A. Jankipersadsing; Floris Imhann; Valerie Collij; Marc Jan Bonder; Xiaofang Jiang; Thomas Gurry; Eric J. Alm; Mauro D'Amato; Rinse K. Weersma; Sicco Scherjon; Cisca Wijmenga; Jingyuan Fu; Alexander Kurilshikov; Alexandra Zhernakova

doi:10.1080/19490976.2018.1528822

Analysis of 1135 gut metagenomes identifies sex-specific resistome profiles

Trishla Sinha, Arnau Vich Vila, Sanzhima Garmaeva, Soesma A. Jankipersadsing, Floris Imhann, Valerie Collij, Marc Jan Bonder, Xiaofang Jiang, Thomas Gurry, Eric J. Alm, Mauro D'Amato, Rinse K. Weersma, Sicco Scherjon, Cisca Wijmenga, Jingyuan Fu, Alexander Kurilshikov, Alexandra Zhernakova^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Several gastrointestinal diseases show a sex imbalance, although the underlying (patho)physiological mechanisms behind this are not well understood. The gut microbiome may be involved in this process, forming a complex interaction with host immune system, sex hormones, medication and other environmental factors. Here we performed sex-specific analyses of fecal microbiota composition in 1135 individuals from a population-based cohort. The overall gut microbiome composition of females and males was significantly different (p = 0.001), with females showing a greater microbial diversity (p = 0.009). After correcting for the effects of intrinsic factors, smoking, diet and medications, female hormonal factors such as the use of oral contraceptives and undergoing an ovariectomy were associated with microbial species and pathways. Females had a higher richness of antibiotic-resistance genes, with the most notable being resistance to the lincosamide nucleotidyltransferase (LNU) gene family. The higher abundance of resistance genes is consistent with the greater prescription of the Macrolide-Lincosamide-Streptogramin classes of antibiotics to females. Furthermore, we observed an increased resistance to aminoglycosides in females with self-reported irritable bowel syndrome. These results throw light upon the effects of common medications that are differentially prescribed between sexes and highlight the importance of sex-specific analysis when studying the gut microbiome and resistome.

Original language	English
Pages (from-to)	358-366
Number of pages	9
Journal	Gut Microbes
Volume	10
Issue number	3
DOIs	https://doi.org/10.1080/19490976.2018.1528822
Publication status	Published - 2019

Keywords

gut microbiome
sex differences
gut resistome
female hormonal factors
ANTIBIOTIC USE
MICROBIOME

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Sinha, T., Vila, A. V., Garmaeva, S., Jankipersadsing, S. A., Imhann, F., Collij, V., Bonder, M. J., Jiang, X., Gurry, T., Alm, E. J., D'Amato, M., Weersma, R. K., Scherjon, S., Wijmenga, C., Fu, J., Kurilshikov, A., & Zhernakova, A. (2019). Analysis of 1135 gut metagenomes identifies sex-specific resistome profiles. Gut Microbes, 10(3), 358-366. https://doi.org/10.1080/19490976.2018.1528822

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title = "Analysis of 1135 gut metagenomes identifies sex-specific resistome profiles",

abstract = "Several gastrointestinal diseases show a sex imbalance, although the underlying (patho)physiological mechanisms behind this are not well understood. The gut microbiome may be involved in this process, forming a complex interaction with host immune system, sex hormones, medication and other environmental factors. Here we performed sex-specific analyses of fecal microbiota composition in 1135 individuals from a population-based cohort. The overall gut microbiome composition of females and males was significantly different (p = 0.001), with females showing a greater microbial diversity (p = 0.009). After correcting for the effects of intrinsic factors, smoking, diet and medications, female hormonal factors such as the use of oral contraceptives and undergoing an ovariectomy were associated with microbial species and pathways. Females had a higher richness of antibiotic-resistance genes, with the most notable being resistance to the lincosamide nucleotidyltransferase (LNU) gene family. The higher abundance of resistance genes is consistent with the greater prescription of the Macrolide-Lincosamide-Streptogramin classes of antibiotics to females. Furthermore, we observed an increased resistance to aminoglycosides in females with self-reported irritable bowel syndrome. These results throw light upon the effects of common medications that are differentially prescribed between sexes and highlight the importance of sex-specific analysis when studying the gut microbiome and resistome.",

keywords = "gut microbiome, sex differences, gut resistome, female hormonal factors, ANTIBIOTIC USE, MICROBIOME",

author = "Trishla Sinha and Vila, {Arnau Vich} and Sanzhima Garmaeva and Jankipersadsing, {Soesma A.} and Floris Imhann and Valerie Collij and Bonder, {Marc Jan} and Xiaofang Jiang and Thomas Gurry and Alm, {Eric J.} and Mauro D'Amato and Weersma, {Rinse K.} and Sicco Scherjon and Cisca Wijmenga and Jingyuan Fu and Alexander Kurilshikov and Alexandra Zhernakova",

year = "2019",

doi = "10.1080/19490976.2018.1528822",

language = "English",

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Sinha, T, Vila, AV, Garmaeva, S , Jankipersadsing, SA , Imhann, F , Collij, V , Bonder, MJ, Jiang, X, Gurry, T, Alm, EJ, D'Amato, M, Weersma, RK, Scherjon, S, Wijmenga, C , Fu, J , Kurilshikov, A & Zhernakova, A 2019, 'Analysis of 1135 gut metagenomes identifies sex-specific resistome profiles', Gut Microbes, vol. 10, no. 3, pp. 358-366. https://doi.org/10.1080/19490976.2018.1528822

TY - JOUR

T1 - Analysis of 1135 gut metagenomes identifies sex-specific resistome profiles

AU - Sinha, Trishla

AU - Vila, Arnau Vich

AU - Garmaeva, Sanzhima

AU - Jankipersadsing, Soesma A.

AU - Imhann, Floris

AU - Collij, Valerie

AU - Bonder, Marc Jan

AU - Jiang, Xiaofang

AU - Gurry, Thomas

AU - Alm, Eric J.

AU - D'Amato, Mauro

AU - Weersma, Rinse K.

AU - Scherjon, Sicco

AU - Wijmenga, Cisca

AU - Fu, Jingyuan

AU - Kurilshikov, Alexander

AU - Zhernakova, Alexandra

PY - 2019

Y1 - 2019

N2 - Several gastrointestinal diseases show a sex imbalance, although the underlying (patho)physiological mechanisms behind this are not well understood. The gut microbiome may be involved in this process, forming a complex interaction with host immune system, sex hormones, medication and other environmental factors. Here we performed sex-specific analyses of fecal microbiota composition in 1135 individuals from a population-based cohort. The overall gut microbiome composition of females and males was significantly different (p = 0.001), with females showing a greater microbial diversity (p = 0.009). After correcting for the effects of intrinsic factors, smoking, diet and medications, female hormonal factors such as the use of oral contraceptives and undergoing an ovariectomy were associated with microbial species and pathways. Females had a higher richness of antibiotic-resistance genes, with the most notable being resistance to the lincosamide nucleotidyltransferase (LNU) gene family. The higher abundance of resistance genes is consistent with the greater prescription of the Macrolide-Lincosamide-Streptogramin classes of antibiotics to females. Furthermore, we observed an increased resistance to aminoglycosides in females with self-reported irritable bowel syndrome. These results throw light upon the effects of common medications that are differentially prescribed between sexes and highlight the importance of sex-specific analysis when studying the gut microbiome and resistome.

AB - Several gastrointestinal diseases show a sex imbalance, although the underlying (patho)physiological mechanisms behind this are not well understood. The gut microbiome may be involved in this process, forming a complex interaction with host immune system, sex hormones, medication and other environmental factors. Here we performed sex-specific analyses of fecal microbiota composition in 1135 individuals from a population-based cohort. The overall gut microbiome composition of females and males was significantly different (p = 0.001), with females showing a greater microbial diversity (p = 0.009). After correcting for the effects of intrinsic factors, smoking, diet and medications, female hormonal factors such as the use of oral contraceptives and undergoing an ovariectomy were associated with microbial species and pathways. Females had a higher richness of antibiotic-resistance genes, with the most notable being resistance to the lincosamide nucleotidyltransferase (LNU) gene family. The higher abundance of resistance genes is consistent with the greater prescription of the Macrolide-Lincosamide-Streptogramin classes of antibiotics to females. Furthermore, we observed an increased resistance to aminoglycosides in females with self-reported irritable bowel syndrome. These results throw light upon the effects of common medications that are differentially prescribed between sexes and highlight the importance of sex-specific analysis when studying the gut microbiome and resistome.

KW - gut microbiome

KW - sex differences

KW - gut resistome

KW - female hormonal factors

KW - ANTIBIOTIC USE

KW - MICROBIOME

U2 - 10.1080/19490976.2018.1528822

DO - 10.1080/19490976.2018.1528822

M3 - Article

C2 - 30373468

SN - 1949-0976

VL - 10

SP - 358

EP - 366

JO - Gut Microbes

JF - Gut Microbes

IS - 3

ER -

Analysis of 1135 gut metagenomes identifies sex-specific resistome profiles

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