Abstract
Preclinical positron emission tomography studies are important to follow disease progression and develop new pharmacological agents. We investigated whether kinetic modeling of 5-HT2A tracer [C-11]MDL 100907 is possible in rats.
Kinetic modeling with either metabolite-corrected plasma curve or with the cerebellum as a reference tissue resulted in a good correlation of nondisplaceable binding potential (BPND) calculated from a two-tissue compartment model (2TCM) or different reference tissue models. Injecting the tracer by a slower bolus decreases the variation in 2TCM outcome parameters and results in a good correlation between k(3)/k(4) and the other models. Application of 0.2 mg/kg cold MDL 100907 resulted in almost complete occupancy of 5-HT2A receptors.
A reference tissue model can be used for [C-11]MDL kinetic modeling in rats, which is preferable in pharmacological or longitudinal studies.
Original language | English |
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Pages (from-to) | 730-738 |
Number of pages | 9 |
Journal | Molecular Imaging and Biology |
Volume | 15 |
Issue number | 6 |
DOIs | |
Publication status | Published - Dec-2013 |
Keywords
- [C-11]MDL 100907
- PET
- Serotonin
- 5-HT2A receptor
- Kinetic modeling
- Reference tissue
- REFERENCE TISSUE MODEL
- HUMAN BRAIN
- CORTEX
- QUANTIFICATION
- BEHAVIOR