Analysis of biopharmaceutical proteins in biological matrices by LC-MS/MS I. Sample preparation

Rainer Bischoff*, Kees J. Bronsema, Nico C. van de Merbel

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)

Abstract

Part I of this review discusses sample-preparation aspects of quantifying biopharmaceutical proteins in complex biological matrices by LC-MS/MS with a focus on blood-derived body fluids. We conclude Part I with a short overview over options for automating the entire analytical procedure, which is indispensable for routine applications in pharmacokinetic and clinical studies. (C) 2013 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)41-51
Number of pages11
JournalTrAC-Trends in Analytical Chemistry
Volume48
DOIs
Publication statusPublished - 2013

Keywords

  • Analytical procedure
  • Automation
  • Biological matrix
  • Biopharmaceuticals
  • Blood-derived body fluid
  • Clinical study
  • Liquid chromatography with tandem mass spectrometry (LC-MS/MS)
  • Pharmacokinetic study
  • Protein
  • Sample preparation
  • TANDEM MASS-SPECTROMETRY
  • PERFORMANCE LIQUID-CHROMATOGRAPHY
  • PEPTIDE IMMUNOAFFINITY ENRICHMENT
  • ABSOLUTE QUANTIFICATION
  • MONOCLONAL-ANTIBODY
  • INTERNAL STANDARD
  • HUMAN SERUM
  • THERAPEUTIC PROTEINS
  • TRYPSIN DIGESTION
  • DEAMIDATION

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