@inbook{f6577d9ca0274ce4b96fa6d613dc9abe,
title = "Analysis of cGMP Signaling in Dictyostelium",
abstract = "Biochemical assays are described to analyze signal transduction by the second messenger cGMP in Dictyostelium. The methods include enzyme assays to measure the activity and regulation of cGMP synthesizing guanylyl cyclases and cGMP-degrading phosphodiesterases. In addition, several methods are described to quantify cGMP levels. The target of cGMP in Dictyostelium is the large protein GbpC that has multiple domains including a Roc domain, a kinase domain, and a cGMP-stimulated Ras-GEF domain. A cGMP-binding assay is described to detect and quantify GbpC.",
keywords = "Dictyostelium/metabolism, Cyclic GMP/metabolism, Signal Transduction, Guanylate Cyclase/metabolism, Protozoan Proteins/metabolism",
author = "{van Haastert}, {Peter J M} and Ineke Keizer-Gunnink and Arjan Kortholt",
note = "{\textcopyright} 2024. The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2024",
month = jul,
day = "3",
doi = "10.1007/978-1-0716-3894-1_13",
language = "English",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press",
pages = "177--194",
editor = "Kimmel, {Alan R.}",
booktitle = "Dictyostelium discoideum",
}