Analysis of different signal peptides for the secretory production of Ama r 2 in gram-positive systems (Lactococcus lactis)

Alireza Vasiee, Neda Norouzi, Farideh Tabatabaee Yazdi, Seyed Ali Mortazavi*, Mojtaba Sankian, Mahmoud Mahmoudi, Fakhri Shahidi

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    Prokaryotic systems have been considered the most affordable and simplest hosts which are being employed to express recombinant proteins such as allergens; nevertheless, without appropriate signal peptide (SP), these systems cannot be used for secretory proteins. Recently, a lot of effort has been put into assessing the potential of gram-positive strains such as lactic acid bacteria for new applications in the production of heterologous proteins. Ama r 2 is a respiratory allergen from Amaranthus retroflexus, whose recombinant production in the probiotic host could be introduced as a specific and effective way to rapid diagnosis and immunotherapy of this allergy. Consequently, the production of this recombinant protein using the prokaryotic system, requires a suitable SP to protect disulfide bonds and to prevent misfolding. This study was designed to predict the best SPs for the expression of Ama r 2 protein in Lactococcus lactis as the host. In this study, 42 signal sequences were selected from SP databases and the most important features of them were evaluated. First, n, h and c regions of the SPs and their probabilities were investigated by signalP software version 4.1. Then, their physicochemical properties were evaluated by Portparam and SOLpro. Moreover, the secretion sorting and sub-cellular localization sites were evaluated by PRED-TAT and ProtcompB software programs. The results revealed that yjgB, entC2 (Entrotoxine type C-2), ent B (Entrotoxine type), blaZ (Beta lactamase), dex (number 21), blm (Beta lactamase 2), dex (Dextranase; number 20) and number 26 were introduced theatrically as the best SPs to express Ama r 2 in Lactococcus lactis.

    Original languageEnglish
    Article number103819
    Number of pages8
    JournalMicrobial Pathogenesis
    Volume138
    DOIs
    Publication statusPublished - Jan-2020

    Keywords

    • Gram positive system
    • Bioinformatics
    • Ama r 2 pollen
    • Signal peptide
    • IN-SILICO
    • HETEROLOGOUS PROTEIN
    • EXPRESSION
    • ALLERGEN
    • POLLEN
    • PREDICTION

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