Analysis of in vivo endothelial cell activation applying RT-PCR following endothelial cell isolation by laser dissection microscopy

SA Asgeirsdottir; Naomi Werner; G Harms; A Van Den Berg; G Molema

Analysis of in vivo endothelial cell activation applying RT-PCR following endothelial cell isolation by laser dissection microscopy

SA Asgeirsdottir^*, Naomi Werner, G Harms, A Van Den Berg, G Molema

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

9 Citations (Scopus)

Abstract

Laser dissection microscopy was applied to isolate endothelial cells from tumors obtained from mice treated with TNF-alpha. RNA integrity was demonstrated from whole sections and dissected cells after acetone fixation and hematoxylin staining. RT-PCR for GAPDH, CD31, VCAM-1, ICAM-1 and E-selectin was successfully performed on these samples. These data demonstrate the feasibility of analyzing local endothelial cell responses in diseased tissues at the level of gene expression.

Original language	English
Title of host publication	CELL SIGNALING, TRANSCRIPTION, AND TRANSLATION AS THERAPEUTIC TARGETS
Editors	M Diederich
Place of Publication	NEW YORK
Publisher	New York Academy of Sciences
Pages	586-589
Number of pages	4
ISBN (Print)	1-57331-428-5
Publication status	Published - 2002
Event	4th International Conference on Cell Signaling, Transcription and Translation as Therapeutic Targets - , Luxembourg Duration: 30-Jan-2002 → 2-Feb-2002

Publication series

Name	ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
Publisher	NEW YORK ACAD SCIENCES
Volume	973
ISSN (Print)	0077-8923

Other

Other	4th International Conference on Cell Signaling, Transcription and Translation as Therapeutic Targets
Country/Territory	Luxembourg
Period	30/01/2002 → 02/02/2002

Keywords

laser dissection microscopy
RT-PCR
endothelial cell
inflammation
angiogenesis
cell adhesion molecules

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Asgeirsdottir, SA., Werner, N., Harms, G., Van Den Berg, A., & Molema, G. (2002). Analysis of in vivo endothelial cell activation applying RT-PCR following endothelial cell isolation by laser dissection microscopy. In M. Diederich (Ed.), CELL SIGNALING, TRANSCRIPTION, AND TRANSLATION AS THERAPEUTIC TARGETS (pp. 586-589). (ANNALS OF THE NEW YORK ACADEMY OF SCIENCES; Vol. 973). New York Academy of Sciences.

Asgeirsdottir, SA ; Werner, Naomi ; Harms, G et al. / Analysis of in vivo endothelial cell activation applying RT-PCR following endothelial cell isolation by laser dissection microscopy. CELL SIGNALING, TRANSCRIPTION, AND TRANSLATION AS THERAPEUTIC TARGETS. editor / M Diederich. NEW YORK : New York Academy of Sciences, 2002. pp. 586-589 (ANNALS OF THE NEW YORK ACADEMY OF SCIENCES).

@inproceedings{0d0cff89c6a14cc889a730c2ce6b3fe6,

title = "Analysis of in vivo endothelial cell activation applying RT-PCR following endothelial cell isolation by laser dissection microscopy",

abstract = "Laser dissection microscopy was applied to isolate endothelial cells from tumors obtained from mice treated with TNF-alpha. RNA integrity was demonstrated from whole sections and dissected cells after acetone fixation and hematoxylin staining. RT-PCR for GAPDH, CD31, VCAM-1, ICAM-1 and E-selectin was successfully performed on these samples. These data demonstrate the feasibility of analyzing local endothelial cell responses in diseased tissues at the level of gene expression.",

keywords = "laser dissection microscopy, RT-PCR, endothelial cell, inflammation, angiogenesis, cell adhesion molecules",

author = "SA Asgeirsdottir and Naomi Werner and G Harms and {Van Den Berg}, A and G Molema",

note = "4th International Conference on Cell Signaling, Transcription and Translation as Therapeutic Targets, 30 January - 2 February 2002, Kirchberg, Luxembourg; 4th International Conference on Cell Signaling, Transcription and Translation as Therapeutic Targets ; Conference date: 30-01-2002 Through 02-02-2002",

year = "2002",

language = "English",

isbn = "1-57331-428-5",

series = "ANNALS OF THE NEW YORK ACADEMY OF SCIENCES",

publisher = "New York Academy of Sciences",

pages = "586--589",

editor = "M Diederich",

booktitle = "CELL SIGNALING, TRANSCRIPTION, AND TRANSLATION AS THERAPEUTIC TARGETS",

}

Asgeirsdottir, SA, Werner, N, Harms, G, Van Den Berg, A & Molema, G 2002, Analysis of in vivo endothelial cell activation applying RT-PCR following endothelial cell isolation by laser dissection microscopy. in M Diederich (ed.), CELL SIGNALING, TRANSCRIPTION, AND TRANSLATION AS THERAPEUTIC TARGETS. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, vol. 973, New York Academy of Sciences, NEW YORK, pp. 586-589, 4th International Conference on Cell Signaling, Transcription and Translation as Therapeutic Targets, Luxembourg, 30/01/2002.

Analysis of in vivo endothelial cell activation applying RT-PCR following endothelial cell isolation by laser dissection microscopy. / Asgeirsdottir, SA; Werner, Naomi; Harms, G et al.
CELL SIGNALING, TRANSCRIPTION, AND TRANSLATION AS THERAPEUTIC TARGETS. ed. / M Diederich. NEW YORK: New York Academy of Sciences, 2002. p. 586-589 (ANNALS OF THE NEW YORK ACADEMY OF SCIENCES; Vol. 973).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Analysis of in vivo endothelial cell activation applying RT-PCR following endothelial cell isolation by laser dissection microscopy

AU - Asgeirsdottir, SA

AU - Werner, Naomi

AU - Harms, G

AU - Van Den Berg, A

AU - Molema, G

N1 - 4th International Conference on Cell Signaling, Transcription and Translation as Therapeutic Targets, 30 January - 2 February 2002, Kirchberg, Luxembourg

PY - 2002

Y1 - 2002

N2 - Laser dissection microscopy was applied to isolate endothelial cells from tumors obtained from mice treated with TNF-alpha. RNA integrity was demonstrated from whole sections and dissected cells after acetone fixation and hematoxylin staining. RT-PCR for GAPDH, CD31, VCAM-1, ICAM-1 and E-selectin was successfully performed on these samples. These data demonstrate the feasibility of analyzing local endothelial cell responses in diseased tissues at the level of gene expression.

AB - Laser dissection microscopy was applied to isolate endothelial cells from tumors obtained from mice treated with TNF-alpha. RNA integrity was demonstrated from whole sections and dissected cells after acetone fixation and hematoxylin staining. RT-PCR for GAPDH, CD31, VCAM-1, ICAM-1 and E-selectin was successfully performed on these samples. These data demonstrate the feasibility of analyzing local endothelial cell responses in diseased tissues at the level of gene expression.

KW - laser dissection microscopy

KW - RT-PCR

KW - endothelial cell

KW - inflammation

KW - angiogenesis

KW - cell adhesion molecules

M3 - Conference contribution

SN - 1-57331-428-5

T3 - ANNALS OF THE NEW YORK ACADEMY OF SCIENCES

SP - 586

EP - 589

BT - CELL SIGNALING, TRANSCRIPTION, AND TRANSLATION AS THERAPEUTIC TARGETS

A2 - Diederich, M

PB - New York Academy of Sciences

CY - NEW YORK

T2 - 4th International Conference on Cell Signaling, Transcription and Translation as Therapeutic Targets

Y2 - 30 January 2002 through 2 February 2002

ER -

Asgeirsdottir SA, Werner N, Harms G, Van Den Berg A, Molema G. Analysis of in vivo endothelial cell activation applying RT-PCR following endothelial cell isolation by laser dissection microscopy. In Diederich M, editor, CELL SIGNALING, TRANSCRIPTION, AND TRANSLATION AS THERAPEUTIC TARGETS. NEW YORK: New York Academy of Sciences. 2002. p. 586-589. (ANNALS OF THE NEW YORK ACADEMY OF SCIENCES).

Analysis of in vivo endothelial cell activation applying RT-PCR following endothelial cell isolation by laser dissection microscopy

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