Analysis of protein dynamics during cytokinesis in budding yeast

S. Okada, C. Wloka, E. Bi*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

4 Citations (Scopus)

Abstract

Cytokinesis is essential for development and survival of all organisms by increasing cell number and diversity. It is a highly regulated process that requires spatiotemporal coordination of hundreds of proteins functioning in the assembly, constriction, and disassembly of a contractile actomyosin ring, targeted vesicle fusion, and localized extracellular matrix remodeling. Cytokinesis has been studied in multiple systems with a wide range of technologies to learn the common principles. In this chapter, we describe the analysis of protein dynamics during cytokinesis in the budding yeast Saccharomyces cerevisiae by several live-cell imaging methods. This, in combination with the power of yeast genetics, has yielded novel insights into the mechanism of cytokinesis. Similar approaches are increasingly used to study this fundamental process in more complex systems.

Original languageEnglish
Title of host publicationCytokinesis
EditorsA Echard
PublisherElsevier
Chapter3
Pages25-45
Number of pages21
Edition1
ISBN (Electronic)978-0-12-809792-2
ISBN (Print)978-0-12-809673-4
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Cell Biology
PublisherElsevier
Volume137
ISSN (Print)0091-679X

Keywords

  • MYOSIN-II
  • SACCHAROMYCES-CEREVISIAE
  • FISSION YEAST
  • CONTRACTILE RING
  • SEPTUM FORMATION
  • ACTIN-RING
  • MITOTIC EXIT
  • CELL-CYCLE
  • POLARITY
  • ACTOMYOSIN

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