Anatomy of a lactococcal phage tail

S McGrath*, H Neve, JFML Seegers, R Eijlander, CS Vegge, L Brondsted, KJ Heller, GF Fitzgerald, FK Vogensen, D van Sinderen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

67 Citations (Scopus)

Abstract

Bacteriophages of the Siphoviridae family utilize a long noncontractile tail to recognize, adsorb to, and inject DNA into their bacterial host. The tail anatomy of the archetypal Siphoviridae X has been well studied, in contrast to phages infecting gram-positive bacteria. This report outlines a detailed anatomical description of a typical member of the Siphoviridae infecting a gram-positive bacterium. The tail superstructure of the lactococcal phage Tuc2009 was investigated using N-terminal protein sequencing, Western blotting, and immunogold transmission electron microscopy, allowing a tangible path to be followed from gene sequence through encoded protein to specific architectural structures on the Tuc2009 virion. This phage displays a striking parity with X with respect to tail structure, which reenforced a model proposed for Tuc2009 tail architecture. Furthermore, comparisons with X and other lactococcal phages allowed the specification of a number of genetic submodules likely to encode specific tail structures.

Original languageEnglish
Pages (from-to)3972-3982
Number of pages11
JournalJournal of Bacteriology
Volume188
Issue number11
DOIs
Publication statusPublished - Jun-2006

Keywords

  • BACTERIOPHAGE-LAMBDA TAIL
  • LACTIC-ACID BACTERIA
  • STRUCTURAL PROTEINS
  • TEMPERATE BACTERIOPHAGE-TP901-1
  • MOLECULAR CHARACTERIZATION
  • TRANSLATIONAL FRAMESHIFT
  • COMPARATIVE GENOMICS
  • DNA-SEQUENCE
  • IDENTIFICATION
  • GENES

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