Anti-oxidative function of follicular fluid HDL and outcomes of modified natural cycle-IVF

Ruxandra A. Nagy, Aafke P. A. van Montfoort, Henk Groen, Irene Homminga, Daniela Andrei, Rima H. Mistry, Josephine L. C. Anderson, Annemieke Hoek, Uwe J. F. Tietge*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

High density lipoproteins (HDL) are the main cholesterol carriers in follicular fluid (FF), the natural environment of oocyte development. Additionally, HDL have critical biological functions such as anti-oxidative capacity, which have not been studied in reproduction. Therefore, this study aimed to investigate whether the anti-oxidative function of FF-HDL is associated with fertility outcomes. From 253 women undergoing modified natural cycle (MNC)-IVF at a single academic centre FF and plasma were collected (n = 375 cycles). Anti-oxidative function of FF was mainly attributable to HDL (n = 8; 83%). FF-HDL had a higher anti-oxidative function than plasma HDL (n = 19, P <0.001) coinciding with increased vitamin E and sphingosine 1 phosphate content (P = 0.028 each). Proteomic analysis indicated no significant differences in major anti-oxidative proteins such as paraoxonase 1, apolipoprotein (apo) A-I or apoA-IV between FF-HDL and matched plasma-HDL (n = 5), while apoC-III, apoE and apoC-II were relatively lower in FF-HDL. Finally, FF-HDL anti-oxidative function was related to a decrease in the odds of the oocyte undergoing normal fertilization, an association that persisted after adjustment for confounders (odds ratio 0.97 (0.93-1), P = 0.041). In conclusion, FF-HDL has considerable anti-oxidative properties that might be relevant for embryo quality.

Original languageEnglish
Article number12817
Number of pages9
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - 6-Sep-2019

Keywords

  • HIGH-DENSITY-LIPOPROTEIN
  • ELEVATED OXIDATIVE STRESS
  • METABOLIC SYNDROME
  • WOMEN
  • MARKERS
  • PLASMA
  • ENDOMETRIOSIS
  • DYSLIPIDEMIA
  • INFERTILITY
  • PARAMETERS

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