Antigen-specific detection of autoantibodies against myeloperoxidase (MPO) and proteinase 3 (PR3)

J. Vanderlocht, J. J.B.C. van Beers, P. C. Limburg, J. Damoiseaux*, C. Roozendaal

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

    4 Citations (Scopus)

    Abstract

    ANCA testing was introduced in many laboratories throughout the world when it was recognized that a significant subset of patients with small vessel vasculopathies presented with such antibodies. Many laboratories developed and introduced in-house testing methods for antigen-specific ANCA detection complementary to indirect immune fluorescence screening. Such in-house tests have proven their merit in diagnosing vasculitis and were important to identify critical steps in the development of antigen-specific assays with high sensitivity and specificity. In the meantime various commercial assays became available for antigen-specific ANCA testing. Because of the high diagnostic accuracy of such assays it can be anticipated that commercial, antigen-specific tests will completely replace in-house testing for MPO- and PR3-ANCA. Furthermore, such tests will replace the need for IIF in the diagnostic workup of AAV. In this light it can be foreseen that the knowledge that underlies the development of in-house ANCA testing will gradually disseminate over time. Therefore we describe the current antigen-specific ANCA ELISAs (direct and capture) with the intention to maintain the knowledge and the identification of the critical steps in the development of robust assays.

    Original languageEnglish
    Title of host publicationAutoantibodies
    Subtitle of host publicationMethods and Protocols
    EditorsGunnar Houen
    PublisherHumana Press
    Pages153-176
    Number of pages24
    DOIs
    Publication statusPublished - 2019

    Publication series

    NameMethods in Molecular Biology
    Volume1901
    ISSN (Print)1064-3745

    Keywords

    • ANCA-associated vasculitis (AAV)
    • Anti-neutrophil cytoplasmic antibody (ANCA)
    • Myeloperoxidase (MPO)
    • Proteinase-3 (PR3)

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