@inbook{64d35c0db8a240b7afa8c150be8a35ac,
title = "Antigen-specific detection of autoantibodies against myeloperoxidase (MPO) and proteinase 3 (PR3)",
abstract = "ANCA testing was introduced in many laboratories throughout the world when it was recognized that a significant subset of patients with small vessel vasculopathies presented with such antibodies. Many laboratories developed and introduced in-house testing methods for antigen-specific ANCA detection complementary to indirect immune fluorescence screening. Such in-house tests have proven their merit in diagnosing vasculitis and were important to identify critical steps in the development of antigen-specific assays with high sensitivity and specificity. In the meantime various commercial assays became available for antigen-specific ANCA testing. Because of the high diagnostic accuracy of such assays it can be anticipated that commercial, antigen-specific tests will completely replace in-house testing for MPO- and PR3-ANCA. Furthermore, such tests will replace the need for IIF in the diagnostic workup of AAV. In this light it can be foreseen that the knowledge that underlies the development of in-house ANCA testing will gradually disseminate over time. Therefore we describe the current antigen-specific ANCA ELISAs (direct and capture) with the intention to maintain the knowledge and the identification of the critical steps in the development of robust assays.",
keywords = "ANCA-associated vasculitis (AAV), Anti-neutrophil cytoplasmic antibody (ANCA), Myeloperoxidase (MPO), Proteinase-3 (PR3)",
author = "J. Vanderlocht and {van Beers}, {J. J.B.C.} and Limburg, {P. C.} and J. Damoiseaux and C. Roozendaal",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
doi = "10.1007/978-1-4939-8949-2_12",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "153--176",
editor = "Gunnar Houen",
booktitle = "Autoantibodies",
}