Abstract
Biocompatible antimicrobial coatings may enhance the function of many orthopedic implants by combating infection. Hydroxyapatite is a choice mineral for such a coating as it is native to bone and silver would be a possible antimicrobial agent as it is also commonly used in biomedical applications. The aim of the research is to develop a silver-containing calcium phosphate (Ag/Ca-P) coating via electrochemical deposition on titanium substrates as this allows for controlled coating buildup on complex shapes and porous surfaces. Two different deposition approaches are explored: one-step Ag/Ca-P(1) deposition coatings, containing silver ions as microsized silver phosphate particles embedded in the Ca-P matrix; and via a two-step method (Ag/Ca-P(2)) where silver is deposited as metallic silver nanoparticle on the Ca-P coating. The Ag/Ca-P(1) coating displays a bacterial reduction of 76.1 +/- 8.3% via Ag-ion leaching. The Ag/Ca-P(2) coating displays a bacterial reduction of 83.7 +/- 4.5% via contact killing. Interestingly, by preincubation in phosphate-buffered saline solution, bacterial reduction improves to 97.6 +/- 2.7 and 99.7 +/- 0.4% for Ag/Ca-P(1) and Ag/Ca-P(2) coatings, respectively, due to leaching of formed AgClx(x-1)- species. The biocompatibility evaluation indicates that the Ag/Ca-P(1) coating is cytotoxic towards osteoblasts while the Ag/Ca-P(2) coating shows excellent compatibility. The electrochemical deposition of highly bactericidal coatings with excellent biocompatibility will enable us to coat future bone implants even with complex or porous structures.
Original language | English |
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Pages (from-to) | 5531-5541 |
Number of pages | 11 |
Journal | ACS Applied Materials & Interfaces |
Volume | 12 |
Issue number | 5 |
Early online date | 14-Jan-2020 |
DOIs | |
Publication status | Published - 5-Feb-2020 |
Keywords
- hydroxyapatite
- electrochemical deposition
- silver nanoparticles
- antimicrobial
- biocompatibility
- HYDROXYAPATITE COMPOSITE COATINGS
- DOPED HYDROXYAPATITE
- ANTIBACTERIAL ACTIVITY
- BIOLOGICAL-PROPERTIES
- IN-VITRO
- NANOPARTICLES
- TITANIUM
- SURFACE
- MECHANISM
- INFECTION