Applications of functional dyes in biomedicine and life sciences

Jingyi Huang

Research output: ThesisThesis fully internal (DIV)

255 Downloads (Pure)

Abstract

Functional dyes are useful tool in Biomedical and Life Sciences. In chapter 2 and 3, we introduced a general and simple design concept using unnatural amino acids (UAAs) as flexible scaffold to link organic fluorophore covalently to a photostabilizer on an arbitrary biomolecular target using only NHS- or click chemistry. With this method, chemically distinct fluorophore species that could not be studied before can be improved through intramolecular photostabilization as “self-healing” fluorophores. . In chapter 4, we developed novel photoswitchable aminoglycosides, that we can call “Opto-Drugs”, which can resist aminoglycoside-modifying enzymes while simultaneously demonstrating selective antibiotic activities upon visible light light irradiation. In chapter 5, we generate an artificial photoresponsive system based on genetically encoded aptameric RNA that can respond to photoisomerizable, cell-permeable small molecules.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • University of Groningen
Supervisors/Advisors
  • Herrmann, Andreas, Supervisor
  • Hofkens, Johan, Assessment committee
  • Arenz, Christoph, Assessment committee, External person
  • Oijen, van, Antoine, Assessment committee
Award date8-May-2020
Place of Publication[Groningen]
Publisher
Print ISBNs978-94-034-2686-0
Electronic ISBNs978-94-034-2687-7
DOIs
Publication statusPublished - 2020

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