Applying broadband terahertz time‐domain spectroscopy to the analysis of crystalline proteins: A dehydration study

Kasia Tych; Andrew D.  Burnett; Christopher D. Wood; John E. Cunningham; Arwen R. Pearson; A.G. Davies; Edmund H. Linfield

doi:10.1107/S0021889810043372

Applying broadband terahertz time‐domain spectroscopy to the analysis of crystalline proteins: A dehydration study

Kasia Tych, Andrew D. Burnett, Christopher D. Wood, John E. Cunningham, Arwen R. Pearson, A.G. Davies, Edmund H. Linfield^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

15 Citations (Scopus)

Abstract

The application of terahertz time‐domain spectroscopy and imaging to the study of proteins in crystalline form is demonstrated. Terahertz time‐domain spectroscopy is particularly sensitive to the long‐range ordering of molecules, with proven utility for the spectroscopy of crystalline biological small molecules. Here, the terahertz time‐domain absorption response of a macromolecular protein single crystal is investigated for the first time. In particular, the effect of dehydration on the terahertz absorption coefficient of tetragonal hen egg white lysozyme crystals is reported.

Original language	English
Pages (from-to)	129-133
Number of pages	5
Journal	Journal of Applied Crystallography
Volume	44
Issue number	1
DOIs	https://doi.org/10.1107/S0021889810043372
Publication status	Published - Feb-2011
Externally published	Yes

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Applying broadband terahertz time-domain spectroscopy to the analysis of crystalline proteins: a dehydration study
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abstract = "The application of terahertz time‐domain spectroscopy and imaging to the study of proteins in crystalline form is demonstrated. Terahertz time‐domain spectroscopy is particularly sensitive to the long‐range ordering of molecules, with proven utility for the spectroscopy of crystalline biological small molecules. Here, the terahertz time‐domain absorption response of a macromolecular protein single crystal is investigated for the first time. In particular, the effect of dehydration on the terahertz absorption coefficient of tetragonal hen egg white lysozyme crystals is reported.",

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AU - Tych, Kasia

AU - Burnett, Andrew D.

AU - Wood, Christopher D.

AU - Cunningham, John E.

AU - Pearson, Arwen R.

AU - Davies, A.G.

AU - Linfield, Edmund H.

PY - 2011/2

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AB - The application of terahertz time‐domain spectroscopy and imaging to the study of proteins in crystalline form is demonstrated. Terahertz time‐domain spectroscopy is particularly sensitive to the long‐range ordering of molecules, with proven utility for the spectroscopy of crystalline biological small molecules. Here, the terahertz time‐domain absorption response of a macromolecular protein single crystal is investigated for the first time. In particular, the effect of dehydration on the terahertz absorption coefficient of tetragonal hen egg white lysozyme crystals is reported.

U2 - 10.1107/S0021889810043372

DO - 10.1107/S0021889810043372

M3 - Article

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JO - Journal of Applied Crystallography

JF - Journal of Applied Crystallography

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Applying broadband terahertz time‐domain spectroscopy to the analysis of crystalline proteins: A dehydration study

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