Arabidopsis AAL-toxin-resistant mutant atr1 shows enhanced tolerance to programmed cell death induced by reactive oxygen species

Tsanko S. Gechev*, MargFiet A. Ferwerda, Nikolay Mehterov, Christophe Laloi, Muhammad K. Qureshi, Jacques Hille

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

The fungal AAL-toxin triggers programmed cell death (PCD) through perturbations of sphingolipid metabolism in AAL-toxin-sensitive plants. While Arabidopsis is relatively insensitive to the toxin, the loh2 mutant exhibits increased Susceptibility to AAL-toxin due to the knockout of a gene involved in sphingolipid metabolism. Genetic screening of mutagenized loh2 seeds resulted in the isolation of AAL-toxin-resistant mutant atr1. Atr1 displays a wild type phenotype when grown on soil but it develops less biomass than loh2 on media supplemented with 2% and 3% sucrose. Atr1 was also more tolerant to the reactive oxygen species-generating herbicides aminotriazole (AT) and paraquat. Microarray analyses of atr1 and loh2 under AT-treatment conditions that trigger cell death in loh2 and no visible damage in atr1 revealed genes specifically regulated in atr1 or loh2. In addition, most of the genes strongly down-regulated in both mutants were related to cell wall extension and cell growth, consistent with the apparent and similar AT-induced cessation of growth in both mutants. This indicates that two different pathways, a first controlling growth inhibition and a second triggering cell death, are associated with AT-induced oxidative stress. (c) 2008 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)639-644
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume375
Issue number4
DOIs
Publication statusPublished - 31-Oct-2008

Keywords

  • AAL-toxin
  • Hydrogen peroxide
  • Reactive oxygen species
  • Sphingolipid metabolism
  • Programmed cell death
  • HYDROGEN-PEROXIDE
  • OXIDATIVE STRESS
  • GENE-EXPRESSION
  • ANTIOXIDANT ENZYMES
  • RESPONSES
  • THALIANA
  • IDENTIFICATION
  • PATHWAYS
  • TOBACCO
  • LIGHT

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