Arabidopsis CPR5 is a senescence-regulatory gene with pleiotropic functions as predicted by the evolutionary theory of senescence

Hai-Chun Jing, Lisa Anderson, Marcel J. G. Sturre, Jacques Hille, Paul P. Dijkwel*

*Corresponding author for this work

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Abstract

Arabidopsis CPR5 is a senescence-regulatory gene with pleiotropic functions as predicted by the evolutionary theory of senescence Hai-Chun Jing1,2, Lisa Anderson3, Marcel J.G. Sturre1, Jacques Hille1 and Paul P. Dijkwel1,* 1Molecular Biology of Plants, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands 2Wheat Pathogenesis Programme, Rothamsted Research, Plant–Pathogen Interaction Division, Harpenden, Herts AL5 2JQ, UK 3Developmental, Cell, and Molecular Biology Group, Department of Biology, Duke University, Durham, NC 27708-1000, USA * Present address and to whom correspondence should be addressed. Institute of Molecular Bioscience, Massey University, Private Bag 11222, Palmenston North, New zealand. E-mail: p.Dijkwel@massey.ac.nz Evolutionary theories of senescence predict that genes with pleiotropic functions are important for senescence regulation. In plants there is no direct molecular genetic test for the existence of such senescence-regulatory genes. Arabidopsis cpr5 mutants exhibit multiple phenotypes including hypersensitivity to various signalling molecules, constitutive expression of pathogen-related genes, abnormal trichome development, spontaneous lesion formation, and accelerated leaf senescence. These indicate that CPR5 is a beneficial gene which controls multiple facets of the Arabidopsis life cycle. Ectopic expression of CPR5 restored all the mutant phenotypes. However, in transgenic plants with increased CPR5 transcripts, accelerated leaf senescence was observed in detached leaves and at late development around 50 d after germination, as illustrated by the earlier onset of senescence-associated physiological and molecular markers. Thus, CPR5 has early-life beneficial effects by repressing cell death and insuring normal plant development, but late-life deleterious effects by promoting developmental senescence. As such, CPR5 appears to function as a typical senescence-regulatory gene as predicted by the evolutionary theories of senescence.
Original languageEnglish
Pages (from-to)3885 - 3894
Number of pages10
JournalJournal of Experimental Botany
Volume58
Issue number14
DOIs
Publication statusPublished - Nov-2007

Keywords

  • Arabidopsis
  • cell death
  • CPR5/OLD1
  • evolutionary senescence
  • hormones
  • leaf senescence
  • MAP-BASED CLONING
  • LEAF SENESCENCE
  • SALICYLIC-ACID
  • DROSOPHILA-MELANOGASTER
  • DEFENSE RESPONSES
  • METHYL JASMONATE
  • GENOME-WIDE
  • PROTEIN
  • RESISTANCE
  • ETHYLENE

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