Arabidopsis RecQl4A suppresses homologous recombination and modulates DNA damage responses

MB Bagherieh-Najjar, Onno M. H. de Vries, Jacques Hille, Paul P. Dijkwel

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Abstract

The DNA damage response and DNA recombination are two interrelated mechanisms involved in maintaining the integrity of the genome, but in plants they are poorly understood. RecO is a family of genes with conserved roles in the regulation of DNA recombination in eukaryotes; there are seven members in Arabidopsis. Here we report on the functional analysis of the Arabidopsis RecQ14A gene. Ectopic expression of Arabidopsis RecQ14A in yeast RecQ-deficient cells suppressed their hypersensitivity to the DNA-damaging drug methyl methanesulfonate (MMS) and enhanced their rate of homologous recombination (HR). Analysis of three recQ14A mutant alleles revealed no obvious developmental defects or telomere deregulation in plants grown under standard growth conditions. Compared with wild-type Arabidopsis, the recQ14A mutant seedlings were found to be hypersensitive to UV light and MMS, and more resistant to mitomycin C. The average frequency of intrachromosomal HR in recQ14A mutant plants was increased 7.5-fold over that observed in wild-type plants. The data reveal roles for Arabidopsis RecQ14A in maintenance of genome stability by modulation of the DNA damage response and suppression of HR.

Original languageEnglish
Pages (from-to)789-798
Number of pages10
JournalPlant Journal
Volume43
Issue number6
DOIs
Publication statusPublished - Sep-2005

Keywords

  • Arabidopsis
  • DNA repair
  • genome instability
  • DNA recombination
  • RecQ
  • ARRESTED REPLICATION FORKS
  • INTERSTRAND CROSS-LINKS
  • DOUBLE-STRAND BREAKS
  • YEAST SGS1 MUTANT
  • SACCHAROMYCES-CEREVISIAE
  • ESCHERICHIA-COLI
  • MAMMALIAN-CELLS
  • TELOMERE MAINTENANCE
  • MMS SENSITIVITY
  • HELICASE

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