Arginine exposure alters ectonucleotidase activities and morphology of zebrafish larvae (Danio rerio)

Katiucia Marques Capiotti, Lidiane Fazenda, Luiza Reali Nazario, Fabiano Peres Menezes, Luiza Wilges Kist, Maurício Reis Bogo, Rosane Souza Da Silva, Angela Terezinha Wyse, Carla Denise Bonan

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20 Citations (Scopus)


Hyperargininemia is an inborn error of metabolism (IEM) characterized by tissue accumulation of arginine (Arg). Mental retardation and other neurological features are common symptoms in hyperargininemic patients. Considering purinergic signaling has a crucial role from the early stages of development and underlying mechanisms of this disease are poorly established, we investigated the effect of Arg administration on locomotor activity, morphological alterations, and extracellular nucleotide hydrolysis in larvae and adult zebrafish. We showed that 0.1 mM Arg was unable to promote changes in locomotor activity. In addition, 7-day-post-fertilization (dpf) larvae treated with Arg demonstrated a decreased body size. Arg exposure (0.1 mM) promoted an increase in ATP, ADP, and AMP hydrolysis when compared to control group. These findings demonstrated that Arg might affect morphological parameters and ectonucleotidase activities in zebrafish larvae, suggesting that purinergic system is a target for neurotoxic effects induced by Arg.

Original languageEnglish
Pages (from-to)75-81
Number of pages7
JournalInternational Journal of Developmental Neuroscience
Issue number1
Publication statusPublished - Feb-2013
Externally publishedYes


  • Adenosine Diphosphate/metabolism
  • Adenosine Monophosphate/metabolism
  • Adenosine Triphosphate/metabolism
  • Age Factors
  • Analysis of Variance
  • Animals
  • Arginine/pharmacology
  • Brain/drug effects
  • Cell Membrane/drug effects
  • Dose-Response Relationship, Drug
  • Gene Expression Regulation, Developmental/drug effects
  • Gene Expression Regulation, Enzymologic/drug effects
  • Larva/drug effects
  • Motor Activity/drug effects
  • Nucleotidases/metabolism
  • Zebrafish

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