Artificial Light‐Harvesting Complexes Enable Rieske Oxygenase Catalyzed Hydroxylations in Non‐Photosynthetic cells

F. Feyza Özgen, Michael E. Runda, Bastien O. Burek, Peter Wied, Jonathan Z. Bloh, Robert Kourist, Sandy Schmidt*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)
26 Downloads (Pure)

Abstract

In this study, we coupled a well-established whole-cell system based on E. coli via light-harvesting complexes to Rieske oxygenase (RO)-catalyzed hydroxylations in vivo. Although these enzymes represent very promising biocatalysts, their practical applicability is hampered by their dependency on NAD(P)H as well as their multicomponent nature and intrinsic instability in cell-free systems. In order to explore the boundaries of E. coli as chassis for artificial photosynthesis, and due to the reported instability of ROs, we used these challenging enzymes as a model system. The light-driven approach relies on light-harvesting complexes such as eosin Y, 5(6)-carboxyeosin, and rose bengal and sacrificial electron donors (EDTA, MOPS, and MES) that were easily taken up by the cells. The obtained product formations of up to 1.3 g L−1 and rates of up to 1.6 mm h−1 demonstrate that this is a comparable approach to typical whole-cell transformations in E. coli. The applicability of this photocatalytic synthesis has been demonstrated and represents the first example of a photoinduced RO system.

Original languageEnglish
Pages (from-to)3982-3987
Number of pages6
JournalAngewandte Chemie International Edition
Volume59
Issue number10
DOIs
Publication statusPublished - 2-Mar-2020
Externally publishedYes

Keywords

  • NAPHTHALENE DIOXYGENASE
  • BIOCATALYTIC OXIDATION
  • ASYMMETRIC REDUCTION
  • ELECTRON-DONORS
  • DRIVEN
  • DIHYDROXYLATION
  • BUFFERS
  • ENZYME
  • C=C

Cite this