Assays to Monitor Autophagy Progression in Cell Cultures

Idil Orhon, Fulvio Reggiori

Research output: Contribution to journalArticleAcademicpeer-review

41 Citations (Scopus)
300 Downloads (Pure)

Abstract

The vast number of implications of autophagy in multiple areas of life sciences and medicine has attracted the interest of numerous scientists that aim to unveil the role of this process in specific physiological and pathological contexts. Cell cultures are one of the most frequently used experimental setup for the investigation of autophagy. As a result, it is essential to assess this highly regulated molecular pathway with efficient and reliable methods. Each method has its own advantages and disadvantages. Here, we present a review summarizing the most established assays used to monitor autophagy induction and progression in cell cultures, in order to guide researchers in the selection of the most optimal solution for their experimental setup and design.

Original languageEnglish
Article number20
Number of pages13
JournalCells
Volume6
Issue number3
DOIs
Publication statusPublished - 7-Jul-2017

Keywords

  • Journal Article
  • Review

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