Assignment of genes to pulse-field separated chromosomes of Schizophyllum commune

Sigrídur A. Ásgeirsdóttir*, Frank H. J. Schuren, Joseph G. H. Wessels

*Corresponding author for this work

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    11 Citations (Scopus)

    Abstract

    Chromosomal DNAs of the basidiomycete Schizophyllum commune were separated by Contour-Clamped Homogeneous Electric Field Electrophoresis (CHEF). The estimated sizes of the chromosomal DNAs ranged from 4.7 Megabase pairs (Mbp) to 1.6 Mbp, totalling 35.6 Mbp. Using sequences from 20 cloned genes we obtained hybridization signals to at least 8 out of 11 chromosomal DNAs. The mating-type genes A and B were mapped to the largest and the smallest chromosomal DNA band, respectively. Developmentally regulated genes which are under the control of the mating-type genes, were localized to five different chromosomal DNA bands.

    Original languageEnglish
    Pages (from-to)689-693
    Number of pages5
    JournalMycological Research
    Volume98
    DOIs
    Publication statusPublished - Jun-1994

    Keywords

    • PHANEROCHAETE-CHRYSOSPORIUM
    • AGARICUS-BISPORUS
    • MESSENGER-RNAS
    • FRUITING GENES
    • AERIAL HYPHAE
    • EXPRESSION
    • KARYOTYPE
    • DNA
    • SEQUENCE
    • DIKARYONS

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