Atg17 regulates the magnitude of the autophagic response

Heesun Cheong, Tomohiro Yorimitsu, Fulvio Reggiori, Julie E Legakis, Chao-Wen Wang, Daniel J Klionsky

Research output: Contribution to journalArticleAcademicpeer-review

192 Citations (Scopus)
76 Downloads (Pure)

Abstract

Autophagy is a catabolic process used by eukaryotic cells for the degradation and recycling of cytosolic proteins and excess or defective organelles. In yeast, autophagy is primarily a response to nutrient limitation, whereas in higher eukaryotes it also plays a role in developmental processes. Due to its essentially unlimited degradative capacity, it is critical that regulatory mechanisms are in place to modulate the timing and magnitude of the autophagic response. One set of proteins that seems to function in this regard includes a complex that contains the Atg1 kinase. Aside from Atg1, the proteins in this complex participate primarily in either nonspecific autophagy or specific types of autophagy, including the cytoplasm to vacuole targeting pathway, which operates under vegetative growth conditions, and peroxisome degradation. Accordingly, these proteins are prime candidates for factors that regulate the conversion between these pathways, including the change in size of the sequestering vesicle, the most obvious morphological difference. The atg17delta mutant forms a reduced number of small autophagosomes. As a result, it is defective in peroxisome degradation and is partially defective for autophagy. Atg17 interacts with both Atg1 and Atg13, via two coiled-coil domains, and these interactions facilitate its inclusion in the Atg1 complex.

Original languageEnglish
Pages (from-to)3438-53
Number of pages16
JournalMolecular Biology of the Cell
Volume16
Issue number7
DOIs
Publication statusPublished - Jul-2005
Externally publishedYes

Keywords

  • Adaptor Proteins, Signal Transducing
  • Alkaline Phosphatase
  • Autophagy
  • Blotting, Western
  • Carrier Proteins
  • Electrophoresis, Polyacrylamide Gel
  • Green Fluorescent Proteins
  • Immunoblotting
  • Microscopy, Fluorescence
  • Models, Biological
  • Mutation
  • Peroxisomes
  • Phagocytosis
  • Phosphoproteins
  • Plasmids
  • Protein Binding
  • Protein Kinases
  • Protein Structure, Tertiary
  • Protein-Serine-Threonine Kinases
  • Saccharomyces cerevisiae Proteins
  • Time Factors
  • Two-Hybrid System Techniques
  • VACUOLE TARGETING PATHWAY
  • TRANSPORT VESICLE FORMATION
  • SACCHAROMYCES-CEREVISIAE
  • SELECTIVE AUTOPHAGY
  • SECRETORY PATHWAY
  • HANSENULA-POLYMORPHA
  • AMINOPEPTIDASE-I
  • YEAST AUTOPHAGY
  • CYTOPLASM
  • PROTEIN

Fingerprint

Dive into the research topics of 'Atg17 regulates the magnitude of the autophagic response'. Together they form a unique fingerprint.

Cite this