Atg21p is essential for macropexophagy and microautophagy in the yeast Hansenula polymorpha

Adriana N Leão-Helder; Arjen M Krikken; Gerd Gellissen; Ida J van der Klei; Marten Veenhuis; Jan A K W Kiel

doi:10.1016/j.febslet.2004.10.055

Atg21p is essential for macropexophagy and microautophagy in the yeast Hansenula polymorpha

Adriana N Leão-Helder, Arjen M Krikken, Gerd Gellissen, Ida J van der Klei, Marten Veenhuis, Jan A K W Kiel

Molecular Cell Biology

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Abstract

ATG genes are required for autophagy-related processes that transport proteins/organelles destined for proteolytic degradation to the vacuole. Here, we describe the identification and characterisation of the Hansenula polymorpha ATG21 gene. Its gene product Hp-Atg21p, fused to eGFP, had a dual location in the cytosol and in peri-vacuolar dots. We demonstrate that Hp-Atg21p is essential for two separate modes of peroxisome degradation, namely glucose-induced macropexophagy and nitrogen limitation-induced microautophagy. In atg21 cells subjected to macropexophagy conditions, sequestration of peroxisomes tagged for degradation is initiated but fails to complete.

Original language	English
Pages (from-to)	491 - 495
Number of pages	5
Journal	FEBS Letters
Volume	577
Issue number	3
DOIs	https://doi.org/10.1016/j.febslet.2004.10.055
Publication status	Published - 2004

Keywords

Autophagy
Cytosol
Fungal Proteins
Genes, Fungal
Glucose
Green Fluorescent Proteins
Microscopy, Fluorescence
Mutation
Peroxisomes
Pichia
Recombinant Fusion Proteins
Saccharomyces cerevisiae Proteins
Vacuoles

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@article{e777369b10f24f3188802094bb64ef7d,

title = "Atg21p is essential for macropexophagy and microautophagy in the yeast Hansenula polymorpha",

abstract = "ATG genes are required for autophagy-related processes that transport proteins/organelles destined for proteolytic degradation to the vacuole. Here, we describe the identification and characterisation of the Hansenula polymorpha ATG21 gene. Its gene product Hp-Atg21p, fused to eGFP, had a dual location in the cytosol and in peri-vacuolar dots. We demonstrate that Hp-Atg21p is essential for two separate modes of peroxisome degradation, namely glucose-induced macropexophagy and nitrogen limitation-induced microautophagy. In atg21 cells subjected to macropexophagy conditions, sequestration of peroxisomes tagged for degradation is initiated but fails to complete.",

keywords = "Autophagy, Cytosol, Fungal Proteins, Genes, Fungal, Glucose, Green Fluorescent Proteins, Microscopy, Fluorescence, Mutation, Peroxisomes, Pichia, Recombinant Fusion Proteins, Saccharomyces cerevisiae Proteins, Vacuoles",

author = "Le{\~a}o-Helder, {Adriana N} and Krikken, {Arjen M} and Gerd Gellissen and {van der Klei}, {Ida J} and Marten Veenhuis and Kiel, {Jan A K W}",

note = "Relation: http://www.rug.nl/gbb/ date_submitted:2007 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute",

year = "2004",

doi = "10.1016/j.febslet.2004.10.055",

language = "English",

volume = "577",

pages = "491 -- 495",

journal = "FEBS Letters",

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TY - JOUR

T1 - Atg21p is essential for macropexophagy and microautophagy in the yeast Hansenula polymorpha

AU - Leão-Helder, Adriana N

AU - Krikken, Arjen M

AU - Gellissen, Gerd

AU - van der Klei, Ida J

AU - Veenhuis, Marten

AU - Kiel, Jan A K W

N1 - Relation: http://www.rug.nl/gbb/ date_submitted:2007 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute

PY - 2004

Y1 - 2004

N2 - ATG genes are required for autophagy-related processes that transport proteins/organelles destined for proteolytic degradation to the vacuole. Here, we describe the identification and characterisation of the Hansenula polymorpha ATG21 gene. Its gene product Hp-Atg21p, fused to eGFP, had a dual location in the cytosol and in peri-vacuolar dots. We demonstrate that Hp-Atg21p is essential for two separate modes of peroxisome degradation, namely glucose-induced macropexophagy and nitrogen limitation-induced microautophagy. In atg21 cells subjected to macropexophagy conditions, sequestration of peroxisomes tagged for degradation is initiated but fails to complete.

AB - ATG genes are required for autophagy-related processes that transport proteins/organelles destined for proteolytic degradation to the vacuole. Here, we describe the identification and characterisation of the Hansenula polymorpha ATG21 gene. Its gene product Hp-Atg21p, fused to eGFP, had a dual location in the cytosol and in peri-vacuolar dots. We demonstrate that Hp-Atg21p is essential for two separate modes of peroxisome degradation, namely glucose-induced macropexophagy and nitrogen limitation-induced microautophagy. In atg21 cells subjected to macropexophagy conditions, sequestration of peroxisomes tagged for degradation is initiated but fails to complete.

KW - Autophagy

KW - Cytosol

KW - Fungal Proteins

KW - Genes, Fungal

KW - Glucose

KW - Green Fluorescent Proteins

KW - Microscopy, Fluorescence

KW - Mutation

KW - Peroxisomes

KW - Pichia

KW - Recombinant Fusion Proteins

KW - Saccharomyces cerevisiae Proteins

KW - Vacuoles

U2 - 10.1016/j.febslet.2004.10.055

DO - 10.1016/j.febslet.2004.10.055

M3 - Article

C2 - 15556634

SN - 0014-5793

VL - 577

SP - 491

EP - 495

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Atg21p is essential for macropexophagy and microautophagy in the yeast Hansenula polymorpha

Abstract

Keywords

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