Atomic composition of the hydrophobic and hydrophilic membrane sides of self-assembled SC3p hydrophobin

HAB Wösten, OMH de Vries, HC van der Mei, HJ BUSSCHER, JGH Wessels*

*Corresponding author for this work

    Research output: Contribution to journalComment/Letter to the editorAcademicpeer-review

    24 Citations (Scopus)


    The hydrophobin SC3p of Schizophyllum commune self-assembles into a 10-nm-thick amphipathic membrane at hydrophilic-hydrophobic interfaces. X-ray photoelectron spectroscopy of the hydrophobic membrane side of SC3p, assembled in vitro, showed an atomic composition similar to the calculated composition of SC3p when glycosylation was taken into account. The atomic composition measured at the hydrophilic membrane side deviated from that at the hydrophobic side and indicated the presence of a lower number of peptide bonds. High levels of S and N were detected only on mycelia carrying hydrophobic aerial hyphae, as expected with assembled SC3p present at the surface of these hyphae.

    Original languageEnglish
    Pages (from-to)7085-7086
    Number of pages2
    JournalJournal of Bacteriology
    Issue number22
    Publication statusPublished - Nov-1994


    • GENES

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