A double imprinting approach was used to generate polymer layers with recognition properties for the detection of atrazine. This straightforward technique enabled us to transfer the biological information of antibodies to plastics. At first, natural antibodies were utilized to pattern the nanoparticles to generate imprints. In a consecutive step, these nanoparticles were assembled onto stamps for surface imprinting the polymer layer to produce replicas. Thus, the generated artificial antibodies show an approximately four times higher sensor response than their natural counterparts. Furthermore, the patterned nanoparticles can be used many times for structuring new sensor layers. Additionally, the synthetic receptors are more robust and allow us to include pre-concentration with organic solvent extraction. Even so, a higher selectivity pattern was observed in case of antibody replicas.