Automated protein crystallization and a new crystal form of a subtilisin: Eglin complex

Henk A. Kelders; Kor H. Kalk; Piet Gros; Wim G. J. Hol

doi:10.1093/protein/1.4.301

Automated protein crystallization and a new crystal form of a subtilisin: Eglin complex

Henk A. Kelders^*, Kor H. Kalk, Piet Gros, Wim G. J. Hol

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

13 Citations (Scopus)

Abstract

A procedure is described for automating labour-intensive steps of the ‘hanging drop’ protein crystallization method. An automatic sample changer is employed to fill the wells in a multi-well plate so that concentration gradients in various components are obtained. The sample changer is also used for preparing droplets on a second multi-well plate. Subse quently, this second plate is manually turned around and placed on top of the first multi-well plate such that a large number of chambers with different conditions is obtained simultaneously. During initial trials a new crystal form of a subtilisin:eglin complex was obtained. The crystals have space group P21 contain two enzyme inhibitor complexes per asymmetric unit and diffract beyond 2.2 Å.

Original language	English
Pages (from-to)	301-303
Number of pages	3
Journal	Protein Engineering
Volume	1
Issue number	4
DOIs	https://doi.org/10.1093/protein/1.4.301
Publication status	Published - 1987

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@article{7c598dca1ee14b039efebe6c25b2b239,

title = "Automated protein crystallization and a new crystal form of a subtilisin: Eglin complex",

abstract = "A procedure is described for automating labour-intensive steps of the {\textquoteleft}hanging drop{\textquoteright} protein crystallization method. An automatic sample changer is employed to fill the wells in a multi-well plate so that concentration gradients in various components are obtained. The sample changer is also used for preparing droplets on a second multi-well plate. Subse quently, this second plate is manually turned around and placed on top of the first multi-well plate such that a large number of chambers with different conditions is obtained simultaneously. During initial trials a new crystal form of a subtilisin:eglin complex was obtained. The crystals have space group P21 contain two enzyme inhibitor complexes per asymmetric unit and diffract beyond 2.2 {\AA}.",

author = "Kelders, {Henk A.} and Kalk, {Kor H.} and Piet Gros and Hol, {Wim G. J.}",

year = "1987",

doi = "10.1093/protein/1.4.301",

language = "English",

volume = "1",

pages = "301--303",

journal = "Protein Engineering",

issn = "0269-2139",

number = "4",

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TY - JOUR

T1 - Automated protein crystallization and a new crystal form of a subtilisin

T2 - Eglin complex

AU - Kelders, Henk A.

AU - Kalk, Kor H.

AU - Gros, Piet

AU - Hol, Wim G. J.

PY - 1987

Y1 - 1987

N2 - A procedure is described for automating labour-intensive steps of the ‘hanging drop’ protein crystallization method. An automatic sample changer is employed to fill the wells in a multi-well plate so that concentration gradients in various components are obtained. The sample changer is also used for preparing droplets on a second multi-well plate. Subse quently, this second plate is manually turned around and placed on top of the first multi-well plate such that a large number of chambers with different conditions is obtained simultaneously. During initial trials a new crystal form of a subtilisin:eglin complex was obtained. The crystals have space group P21 contain two enzyme inhibitor complexes per asymmetric unit and diffract beyond 2.2 Å.

AB - A procedure is described for automating labour-intensive steps of the ‘hanging drop’ protein crystallization method. An automatic sample changer is employed to fill the wells in a multi-well plate so that concentration gradients in various components are obtained. The sample changer is also used for preparing droplets on a second multi-well plate. Subse quently, this second plate is manually turned around and placed on top of the first multi-well plate such that a large number of chambers with different conditions is obtained simultaneously. During initial trials a new crystal form of a subtilisin:eglin complex was obtained. The crystals have space group P21 contain two enzyme inhibitor complexes per asymmetric unit and diffract beyond 2.2 Å.

U2 - 10.1093/protein/1.4.301

DO - 10.1093/protein/1.4.301

M3 - Article

SN - 0269-2139

VL - 1

SP - 301

EP - 303

JO - Protein Engineering

JF - Protein Engineering

IS - 4

ER -

Automated protein crystallization and a new crystal form of a subtilisin: Eglin complex

Abstract

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