Autophagy stimulated proliferation of porcine PSCs might be regulated by the canonical Wnt signaling pathway

Lipeng Ren, Wei Han, Hong Yang, Fen Sun, Shuanshuan Xu, Shuxian Hu, Mingzhi Zhang, Xin He, Jinlian Hua, Sha Peng

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

Porcine pancreatic stem cells (PSCs) are one kind of the potential cells for treatment of human diabetes. Autophagy is a highly conserved cellular degradation process in which it helps to maintain the balance between the synthesis, degradation and subsequent recycling of cellular components. However, how autophagy contributes to PSCs has not yet been investigated. Here, we established GFP-LC3 transfected porcine PSC lines in which the accumulation of autophagosomes can be efficiently visualized to evaluate the autophagic activity. Moreover, we observed that starved PSCs which showed increased autophagic activity exhibited an increased tendency to proliferate through the results of BrdU, flow cytometry and western blotting. Furthermore, increased expression of active β-catenin after inducing autophagy indicated that it might be the canonical Wnt signaling that autophagy activated to exert the function on the stimulation of PSCs proliferation. Collectively, these results demonstrated that autophagy stimulated proliferation of PSCs might be regulated by the canonical Wnt signaling pathway. Our results for the first time shed light on a role of autophagy for stimulating the proliferation of porcine PSCs.
Original languageEnglish
Pages (from-to)537-543
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume479
Issue number3
DOIs
Publication statusPublished - 21-Oct-2016
Externally publishedYes

Keywords

  • Autophagy
  • Diabetes
  • Pancreatic stem cells (PSCs)
  • Proliferation
  • Wnt signaling
  • BETA-CATENIN
  • CELLS
  • DIFFERENTIATION
  • DEGRADATION

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