A straightforward and transparent model, based on Gaussian beam optics, for the axial ro resolution of a confocal microscope is presented, A confocal Raman microscope was used to determine the axial confocality in practice. The axial response of a thin planar object was measured for three different objectives, two pinhole sizes and a slit. The results show that, in the case of a confocal configuration, the response calculated with the model provides a good prediction of the axial resolution of the confocal microscope.
|Number of pages||7|
|Journal||Journal of Microscopy|
|Publication status||Published - Dec-1997|