Axial resolution of confocal Raman microscopes: Gaussian beam theory and practice

C J De Grauw, N M Sijtsema, C Otto, J Greve

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    Abstract

    A straightforward and transparent model, based on Gaussian beam optics, for the axial ro resolution of a confocal microscope is presented, A confocal Raman microscope was used to determine the axial confocality in practice. The axial response of a thin planar object was measured for three different objectives, two pinhole sizes and a slit. The results show that, in the case of a confocal configuration, the response calculated with the model provides a good prediction of the axial resolution of the confocal microscope.
    Original languageEnglish
    Pages (from-to)273-279
    Number of pages7
    JournalJournal of Microscopy
    Volume188
    DOIs
    Publication statusPublished - Dec-1997

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