Bacillus subtilis Biosensor Engineered To Assess Meat Spoilage

Alicja Daszczuk, Yonathan Dessalegne, Ismael Drenth, Elbrich Hendriks, Emeraldo Jo, Tom van Lente, Arjan Oldebesten, Jonathon Parrish, Wlada Poljakova, Annisa A. Purwanto, Renske van Raaphorst, Mirjam Boonstra, Auke van Heel, Martijn Herber, Sjoerd van der Meulen, Jeroen Siebring, Robin A. Sorg, Matthias Heinemann*, Oscar P. Kuipers, Jan-Willem Veening

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)

Abstract

Here, we developed a cell-based biosensor that can assess meat freshness using the Gram-positive model bacterium Bacillus subtilis as a chassis. Using transcriptome analysis, we identified promoters that are specifically activated by volatiles released from spoiled meat. The most strongly activated promoter was PsboA, which drives expression of the genes required for the bacteriocin subtilosin. Next, we created a novel BioBrick compatible integration plasmid for B. subtilis and cloned PsboA as a BioBrick in front of the gene encoding the chromoprotein amilGFP inside this vector. We show that the newly identified promoter could efficiently drive fluorescent protein production in B. subtilis in response to spoiled meat and thus can be used as a biosensor to detect meat spoilage.

Original languageEnglish
Pages (from-to)999-1002
Number of pages4
JournalACS Synthetic Biology
Volume3
Issue number12
DOIs
Publication statusPublished - 19-Dec-2014

Keywords

  • GENES

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