Bacillus subtilis YqjG is required for genetic competence development

Manfred J. Saller, Andreas Otto, Greetje A. Berrelkamp-Lahpor, Doerte Becher, Michael Hecker, Arnold J. M. Driessen*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

19 Citations (Scopus)
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Abstract

Members of the evolutionary conserved Oxa1/Alb3/YidC family have been shown to play an important role in membrane protein insertion, folding and/or assembly. Bacillus subtilis contains two YidC-like proteins, denoted as SpoIIIJ and YqjG. SpoIIIJ and YqjG are largely exchangeable, but SpoIIIJ is essential for spore formation and YqjG cannot complement this activity. To elucidate the role of YqjG, we determined the membrane proteome and functional aspects of B. subtilis cells devoid of SpoIIIJ, YqjG or both. The data show that SpoIIIJ and YqjG have complementary functions in membrane protein insertion and assembly. The reduced levels of F1FO ATP synthase in cells devoid of both SpoIIIJ and YqjG are due to a defective assembly of the F-1-domain onto the F-0-domain. Importantly, for the first time, a specific function is demonstrated for YqjG in genetic competence development.

Original languageEnglish
Pages (from-to)270-282
Number of pages13
JournalProteomics
Volume11
Issue number2
DOIs
Publication statusPublished - Jan-2011

Keywords

  • Bacillus subtilis
  • Functional proteomics
  • Membrane proteins
  • Microbiology
  • Phage shock protein A
  • YidC
  • MEMBRANE-PROTEIN BIOGENESIS
  • ESCHERICHIA-COLI YIDC
  • QUANTITATIVE-ANALYSIS
  • BACTERIAL YIDC
  • INNER MEMBRANE
  • SPOIIIJ GENE
  • EXPRESSION
  • INSERTION
  • SPORULATION
  • SECRETION

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