Bacteria-targeted infection imaging: Towards a faster diagnosis of bacterial infection

    Research output: ThesisThesis fully internal (DIV)

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    Abstract

    Bacterial infections occur frequently and are a major threat to human health, causing high morbidity and mortality all over the world. For adequate treatment of infections, a rapid and precise diagnosis is imperative. Presently, this is achieved via microbiological culture and molecular detection, which are often time-consuming processes. Moreover, it is not always possible to obtain appropriate material for culturing, which complicates the diagnostic process. Therefore, the PhD research described in this thesis was aimed at exploring imaging-based techniques for faster and preferably real-time diagnosis of infectious diseases, and for rapid distinction of bacterial infection from sterile inflammation. To this end, the development of new bacteria-targeted imaging modalities was reviewed with a special focus on bacteria-targeted imaging with fluorescent tracers. Subsequently, the possible use of different tracers for infection imaging was experimentally explored. The results show that imaging with fluorescent bacteria-targeted tracers can be readily applied to detect infection ex vivo, by arthroscopy or bronchoscopy, or intra-operatively. Altogether, it is concluded that bacteria-targeted fluorescence imaging approaches may allow faster, real-time diagnosis of bacterial infections.
    Original languageEnglish
    QualificationDoctor of Philosophy
    Awarding Institution
    • University of Groningen
    Supervisors/Advisors
    • van Dijl, Jan Maarten, Supervisor
    • van Dam, Go, Supervisor
    • van Oosten, Marleen, Co-supervisor
    Award date8-Sep-2021
    Place of Publication[Groningen]
    Publisher
    DOIs
    Publication statusPublished - 2021

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