Bacterial protein translocation: kinetic and thermodynamic role of ATP and the protonmotive force

Arnold J.M. Driessen

doi:10.1016/0968-0004(92)90381-I

Bacterial protein translocation: kinetic and thermodynamic role of ATP and the protonmotive force

Arnold J.M. Driessen

Research output: Contribution to journal › Review article › peer-review

42 Citations (Scopus)

Abstract

The energetic mechanism of preprotein export in Escherichia coli has been a source of controversy for many years. In vitro studies of translocation reactions that use purified soluble and membrane components have now clarified the main features of this mechanism. Translocation occurs through consecutive steps which each have distinct energy requirements. Initiation of translocation requires ATP and the SecA protein. Most of the further steps can be driven by the protonmotive force (DELTA-p).

Original language	English
Pages (from-to)	219-223
Number of pages	5
Journal	Trends in Biochemical Sciences
Volume	17
Issue number	6
DOIs	https://doi.org/10.1016/0968-0004(92)90381-I
Publication status	Published - Jun-1992

Keywords

COLI PLASMA-MEMBRANE
ESCHERICHIA-COLI
SECA PROTEIN
MOTIVE FORCE
SECRETION
VESICLES
INTERMEDIATE
SECY/E
OMPA

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Bacterial protein translocation kinetic and thermodynamic role of ATP and the protonmotive force
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@article{cd19a584d8e84c42a9e63e0b0a904d47,

title = "Bacterial protein translocation: kinetic and thermodynamic role of ATP and the protonmotive force",

abstract = "The energetic mechanism of preprotein export in Escherichia coli has been a source of controversy for many years. In vitro studies of translocation reactions that use purified soluble and membrane components have now clarified the main features of this mechanism. Translocation occurs through consecutive steps which each have distinct energy requirements. Initiation of translocation requires ATP and the SecA protein. Most of the further steps can be driven by the protonmotive force (DELTA-p).",

keywords = "COLI PLASMA-MEMBRANE, ESCHERICHIA-COLI, SECA PROTEIN, MOTIVE FORCE, SECRETION, VESICLES, INTERMEDIATE, SECY/E, OMPA",

author = "Driessen, {Arnold J.M.}",

note = "Relation: http://www.rug.nl/gbb/ date_submitted:2007 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute",

year = "1992",

month = jun,

doi = "10.1016/0968-0004(92)90381-I",

language = "English",

volume = "17",

pages = "219--223",

journal = "Trends in Biochemical Sciences",

issn = "0968-0004",

publisher = "ELSEVIER SCIENCE LONDON",

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TY - JOUR

T1 - Bacterial protein translocation

T2 - kinetic and thermodynamic role of ATP and the protonmotive force

AU - Driessen, Arnold J.M.

N1 - Relation: http://www.rug.nl/gbb/ date_submitted:2007 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute

PY - 1992/6

Y1 - 1992/6

N2 - The energetic mechanism of preprotein export in Escherichia coli has been a source of controversy for many years. In vitro studies of translocation reactions that use purified soluble and membrane components have now clarified the main features of this mechanism. Translocation occurs through consecutive steps which each have distinct energy requirements. Initiation of translocation requires ATP and the SecA protein. Most of the further steps can be driven by the protonmotive force (DELTA-p).

AB - The energetic mechanism of preprotein export in Escherichia coli has been a source of controversy for many years. In vitro studies of translocation reactions that use purified soluble and membrane components have now clarified the main features of this mechanism. Translocation occurs through consecutive steps which each have distinct energy requirements. Initiation of translocation requires ATP and the SecA protein. Most of the further steps can be driven by the protonmotive force (DELTA-p).

KW - COLI PLASMA-MEMBRANE

KW - ESCHERICHIA-COLI

KW - SECA PROTEIN

KW - MOTIVE FORCE

KW - SECRETION

KW - VESICLES

KW - INTERMEDIATE

KW - SECY/E

KW - OMPA

U2 - 10.1016/0968-0004(92)90381-I

DO - 10.1016/0968-0004(92)90381-I

M3 - Review article

SN - 0968-0004

VL - 17

SP - 219

EP - 223

JO - Trends in Biochemical Sciences

JF - Trends in Biochemical Sciences

IS - 6

ER -