BALB/c and C57BL/6 Mice Differ in Polyreactive IgA Abundance, which Impacts the Generation of Antigen-Specific IgA and Microbiota Diversity

Floris Fransen, Elena Zagato, Elisa Mazzini, Bruno Fosso, Caterina Manzari, Sahar El Aidy, Andrea Chiavelli, Anna Maria D'Erchia, Maya K Sethi, Oliver Pabst, Marinella Marzano, Silvia Moretti, Luigina Romani, Giuseppe Penna, Graziano Pesole, Maria Rescigno

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131 Citations (Scopus)

Abstract

The interrelationship between IgAs and microbiota diversity is still unclear. Here we show that BALB/c mice had higher abundance and diversity of IgAs than C57BL/6 mice and that this correlated with increased microbiota diversity. We show that polyreactive IgAs mediated the entrance of non-invasive bacteria to Peyer's patches, independently of CX3CR1(+) phagocytes. This allowed the induction of bacteria-specific IgA and the establishment of a positive feedback loop of IgA production. Cohousing of mice or fecal transplantation had little or no influence on IgA production and had only partial impact on microbiota composition. Germ-free BALB/c, but not C57BL/6, mice already had polyreactive IgAs that influenced microbiota diversity and selection after colonization. Together, these data suggest that genetic predisposition to produce polyreactive IgAs has a strong impact on the generation of antigen-specific IgAs and the selection and maintenance of microbiota diversity.

Original languageEnglish
Pages (from-to)527-540
Number of pages14
JournalImmunity
Volume43
Issue number3
DOIs
Publication statusPublished - 15-Sep-2015

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