Biocatalytic self-assembly of 2D peptide-based nanostructures

Meghan Hughes, Haixia Xu, Pim W. J. M. Frederix, Andrew M. Smith, Neil T. Hunt, Tell Tuttle, Ian A. Kinloch, Rein V. Ulijn*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

49 Citations (Scopus)

Abstract

Peptide based 2D nanostructures of micronscale size in both X and Y dimensions are extremely rare because amino acid chirality favours helical structures, and nucleation-growth mechanisms usually favour uni-directional growth. We demonstrate the production of extended two-dimensional (2D) peptide nanostructures via the thermolysin catalysed condensation of Fmoc protected hydrophilic amino acid (serine, Fmoc-S) and a hydrophobic amino acid ester (phenylalanine, F-OMe). We propose that lateral self-assembly is enabled by the reversible nature of the system, favouring the thermodynamic product (extended sheets) over kinetically favoured 1 dimensional structures. Fmoc-SF-OMe forms extended arrays of beta-sheet structures interlock via pi-stacking between Fmoc groups. We propose that, due to its alternating hydrophilic/hydrophobic amino acid sequence, amphiphilic sheets presenting either phenyl or hydroxyl functionality are formed that assemble pair-wise, thereby shielding hydrophobic groups from the aqueous environment. Formation of these structures was supported by fluorescence emission spectroscopy, FTIR and XRD analysis and molecular mechanics minimization. At enhanced enzyme concentrations, hierarchical self-assembly was observed giving rise to spherulitic structures, with the number of spherulites dictated by enzyme concentration.

Original languageEnglish
Pages (from-to)10032-10038
Number of pages7
JournalSoft Matter
Volume7
Issue number21
DOIs
Publication statusPublished - 2011
Externally publishedYes

Keywords

  • SUPRAMOLECULAR HYDROGELS
  • NANOPARTICLE ARRAYS
  • METALLIC NANOWIRES
  • MOLECULAR-DYNAMICS
  • DIPEPTIDE
  • ARCHITECTURE
  • NANOTUBES
  • SELECTION
  • GELATION
  • DESIGN

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