Biochemistry and genetics of inositol phosphate metabolism in Dictyostelium

PJM vanHaastert*, P. van Dijken

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

20 Citations (Scopus)

Abstract

Biochemical and genetic data on the metabolism of inositol phosphates in the microorganism Dictyostelium are combined in a scheme composed of in five subroutes. The first subroute is the inositol cycle as found in other organisms:inositol is incorporated into phospholipids that are hydrolysed by PLC producing Ins(1,4,5)P-3 which is dephosphorylated to inositol, The second subroute is the sequential phosphorylation of inositol to InsP(6); the Ins(3,4,6)P-3 intermediate does not release Ca2+. The third subroute is the sequential phosphorylation of Ins(1,4,5)P-3 to InsP(6) in a nucleus associated fraction, whereas the fourth subroute is the dephosphorylation of Ins(1,3,4,5,6)P-5 to Ins(1,4,5)P-3 at the plasma membrane, This last route mediates Ins(1,4,5)P-3 formation in cells with a disruption of the single PLC gene. Finally, we recognize the formation of InsP(7) and InsP(8) as the fifth subroute. (C) 1997 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)39-43
Number of pages5
JournalFEBS Letters
Volume410
Issue number1
DOIs
Publication statusPublished - 23-Jun-1997
EventAmsterdam FEBS Special Meeting on Cell Signalling Mechanisms - , Netherlands
Duration: 30-Jun-19972-Jul-1997

Keywords

  • Ins(1,4,5)P-3
  • phospholipase C
  • cloning
  • chemotaxis
  • signal transduction
  • PHOSPHOLIPASE-C
  • DEVELOPMENTAL REGULATION
  • G-PROTEINS
  • DISCOIDEUM
  • 1,4,5-TRISPHOSPHATE
  • RECEPTORS
  • 1,3,4,5-TETRAKISPHOSPHATE
  • ACCUMULATION
  • KINASE
  • ENZYME

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