Bioinspired Silica Mineralization on Viral Templates

Christina Dickmeis, Klara Altintoprak, Patrick van Rijn, Christina Wege, Ulrich Commandeur

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic

11 Citations (Scopus)

Abstract

Plant virus capsids are attractive entities for nanotechnological applications because of their variation in shape and natural assembly ability. This chapter describes the production and modification of three differently shaped plant virus capsids for silica mineralization purposes. The chosen plant viruses exhibit either an icosahedral (cowpea mosaic virus, CPMV), or a flexuous rod-like structure (potato virus X, PVX), or a rigid rod-like shape (tobacco mosaic virus, TMV), and are well-known and frequently used plant viruses for biotechnological applications. We describe the production (including genetic or chemical modification) and purification of the plant viruses or of empty virus-like particles in the case of CPMV, as well as the characterization of these harvested templates. The mineralization procedures and differences in the protocols specific to the distinct viruses are described, and the analyses of the mineralization results are explained.

Original languageEnglish
Title of host publicationVirus-Derived Nanoparticles for Advanced Technologies
Subtitle of host publicationMethods and Protocols
EditorsChristina Wege, George P. Lomonossoff
Place of PublicationNew York
PublisherHumana Press
Pages337-362
Number of pages26
ISBN (Electronic)978-1-4939-7808-3
ISBN (Print)978-1-4939-7806-9
DOIs
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

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