Bioluminescence as Gold Standard for Validation of Optical Imaging Modalities in Peritoneal Carcinomatosis Animal Models

N. J. Harlaar, J. W. Hesselink, J. S. de Jong, G. M. van Dam*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

Background: The outcome of cytoreductive surgery in patients with peritoneal carcinomatosis is influenced by incomplete resection as a result of inadequate detection of a tumor, i.e. residual disease. The future perspective of complete resection, made possible by application of intraoperative near-infrared fluorescence imaging (NIRF), led to the development and validation of a bioluminescent colorectal peritoneal carcinomatosis xenograft rat model to act as the gold standard for the evaluation of new optical imaging modalities. Methods: Twenty nude rats were inoculated intraperitoneally with 2 x 10(6) luciferase-labeled human colorectal tumor cells (HT-29-luc-D6). The peritoneal carcinomatosis index (PCI) was estimated using visual observation (PCI-VO) and VO combined with bioluminescence imaging (PCI-BLI). Subsequently, the BL images were presented, and residual tumor tissue was localized by PCI-BLI scoring and compared with the PCI-VO. Results: BLI revealed additional tumor tissue, confirmed by HE staining, compared to VO alone in 7 out of 8 rats (p <0.02). Conclusion: The developed model turned out to be suitable. The use of BLI for tumor detection was more sensitive compared to VO alone. In this model, BLI significantly detected residual disease, and therefore, BLI can be denominated as the gold standard for the evaluation of optical imaging modalities like NIRF. Copyright (C) 2010 S. Karger AG, Basel

Original languageEnglish
Pages (from-to)308-313
Number of pages6
JournalEuropean surgical research
Volume45
Issue number3-4
DOIs
Publication statusPublished - 2010

Keywords

  • Intraoperative imaging
  • Near-infrared fluorescence
  • Bioluminescence imaging
  • Peritoneal carcinomatosis
  • Hyperthermic intraperitoneal chemotherapy
  • Peritoneal carcinomatosis index
  • INTRAPERITONEAL CHEMOTHERAPY
  • COLORECTAL ORIGIN
  • CYTOREDUCTIVE SURGERY
  • IN-VIVO
  • CANCER
  • SURVIVAL
  • MICE
  • METASTASES
  • COLON

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