Abstract
The cytoskeleton is an actively regulated complex network in the cell. One of the most researched components is actin. In our work we developed and tested two microfluidic systems both being applicable to construct quasi 2-dimensional biomimetic actin networks. The first system uses polydimethylsiloxane micropillars, the other polystyrene microparticles held by holographic optical tweezers as anchoring points. Our devices provide actin networks with mesh sizes from a few micrometers up to the order of 10 micrometers. Qualitative analysis shows similar network formation in both systems. Crosslinking was tested using filamin, α-actinin, Ca and Mg ions. The crosslinking process is characterized by a zipping like event, which is limited only by the high stretching modulus of the actin filaments. © 2009 World Scientific Publishing Company.
| Original language | English |
|---|---|
| Pages (from-to) | 17-32 |
| Number of pages | 16 |
| Journal | Biophysical Reviews and Letters |
| Volume | 4 |
| Issue number | 1-2 |
| Publication status | Published - 21-Jul-2009 |
Keywords
- Actin
- Alpha-actinin
- Cortex model
- Crosslinking
- Filamin
- Holographic optical tweezer
- Microfluidics
- Micropillar
- alpha actinin
- calcium ion
- dimeticone
- F actin
- filamin
- G actin
- magnesium ion
- polystyrene
- actin filament
- article
- binding kinetics
- biomimetics
- controlled study
- cross linking
- cytoskeleton
- microfluidics
- molecular dynamics
- molecular mechanics
- optical tweezers
- priority journal
- qualitative analysis
- Young modulus