Biomimetic Multiscale Hierarchical Topography Enhances Osteogenic Differentiation of Human Mesenchymal Stem Cells

Yang Liangliang, Ge Lu, Zhou Qihui, Taraneh Mokabber, Pei Yutao, Reinier Bron, Patrick van Rijn*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)
33 Downloads (Pure)

Abstract

The interface between materials and cells plays a critical role in many biomedical applications. Inspired by the hierarchical architecture of collagen, most abundant structure in the extracellular matrix (ECM), a multiscale hierarchical topography is designed to mimic the collagen nano/micro hierarchical topography. It is hypothesized that the ECM topography affects osteogenesis of human mesenchymal stem cells but until now, it cannot be studied without the biochemical and mechanical influences of the ECM. The multiscale hierarchical topography is achieved by innovatively using sequentially aligned topography preparation via a silicone stretch-oxidation-release method and imprinting lithography. The anisotropically hierarchical topography influences stem cell morphology, orientation, and osteogenic differentiation. Intriguingly, the design resembling that of assembled collagen, exhibits the highest degree of osteogenesis. The hierarchical topotaxis effects are further exemplified by the enhanced vinculin expression, cell contractility, and more pronounced nuclear translocation of Yes-associated protein with the collagen-mimicking topography, indicative for enhanced osteogenesis. The developed multiscale hierarchical system provides insights into the importance of specific biological ECM-like topography by decoupling the biochemical influence. Various diseases, cancer, osteoarthritis, and fibrosis display impaired ECM structures, and therefore this system may have a great potential for tissue engineering approaches and developing in vitro disease models.

Original languageEnglish
Article number2000385
Number of pages12
JournalAdvanced Materials Interfaces
Volume7
Issue number14
DOIs
Publication statusE-pub ahead of print - 13-May-2020

Keywords

  • collagen
  • extracellular matrix
  • hierarchical topography
  • human mesenchymal stem cells
  • mechanotransduction
  • osteogenic differentiation
  • FOCAL ADHESION
  • SURFACE-ROUGHNESS
  • ENDOTHELIAL-CELLS
  • CONTACT GUIDANCE
  • NANOTOPOGRAPHY
  • TISSUE
  • STIFFNESS
  • HYDROGEL
  • YAP/TAZ
  • BIOMATERIALS

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