Biosensing systems for the detection and quantification of methane gas

Noemi Poma, Andrea Bonini, Federico Vivaldi, Denise Biagini, Mariagrazia Di Luca, Daria Bottai, Fabio Di Francesco, Arianna Tavanti*

*Corresponding author for this work

Research output: Contribution to journalShort surveypeer-review

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Abstract

Abstract: Climate change due to the continuous increase in the release of green-house gasses associated with anthropogenic activity has made a significant impact on the sustainability of life on our planet. Methane (CH4) is a green-house gas whose concentrations in the atmosphere are on the rise. CH4 measurement is important for both the environment and the safety at the industrial and household level. Methanotrophs are distinguished for their unique characteristic of using CH4 as the sole source of carbon and energy, due to the presence of the methane monooxygenases that oxidize CH4 under ambient temperature conditions. This has attracted interest in the use of methanotrophs in biotechnological applications as well as in the development of biosensing systems for CH4 quantification and monitoring. Biosensing systems using methanotrophs rely on the use of whole microbial cells that oxidize CH4 in presence of O2, so that the CH4 concentration is determined in an indirect manner by measuring the decrease of O2 level in the system. Although several biological properties of methanotrophic microorganisms still need to be characterized, different studies have demonstrated the feasibility of the use of methanotrophs in CH4 measurement. This review summarizes the contributions in methane biosensing systems and presents a prospective of the valid use of methanotrophs in this field. Key points: • Methanotroph environmental relevance in methane oxidation • Methanotroph biotechnological application in the field of biosensing • Methane monooxygenase as a feasible biorecognition element in biosensors.

Original languageEnglish
Pages (from-to)5627-5634
Number of pages8
JournalApplied Microbiology and Biotechnology
Volume107
Issue number18
DOIs
Publication statusPublished - Sept-2023

Keywords

  • Biosensors
  • Methane biosensing
  • Methane monooxygenase
  • Methanotrophs

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