Blending Baeyer-Villiger monooxygenases: using a robust BVMO as a scaffold for creating chimeric enzymes with novel catalytic properties

Hugo L. van Beek, Gonzalo de Gonzalo, Marco W. Fraaije*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

50 Citations (Scopus)

Abstract

The thermostable Baeyer-Villiger monooxygenase (BVMO) phenylacetone monooxygenase (PAMO) is used as a scaffold to introduce novel selectivities from other BVMOs or the metagenome by structure-inspired subdomain exchanges. This yields biocatalysts with new preferences in the oxidation of sulfides and the Baeyer-Villiger oxidation of ketones, all while maintaining most of the original thermostability.

Original languageEnglish
Pages (from-to)3288-3290
Number of pages3
JournalChemical Communications
Volume48
Issue number27
DOIs
Publication statusPublished - 2012

Keywords

  • DIRECTED EVOLUTION
  • PHENYLACETONE MONOOXYGENASE
  • OXIDATION
  • GENOME

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