Bryophyte DNA sequences from faeces of an arctic herbivore, barnacle goose (Branta leucopsis)

M. Stech*, E. Kolvoort, M. J. J. E. Loonen, K. Vrieling, J. D. Kruijer

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

We tested DNA extraction methods and PCR conditions for the amplification of bryophyte DNA from barnacle goose (Branta leucopsis) faeces collected from Spitsbergen (Svalbard). Both the Qiagen stool kit and a silica-based extraction method received sufficient DNA from fresh and older droppings, as indicated by successful amplification of the plastid psbA-trnH spacer. Standard Taq polymerase outperformed two hot start polymerases. Sequencing of cloned PCR products revealed at least ten moss and two angiosperm sequences. This first example of identifying bryophyte DNA from faeces will allow analysing moss diets of arctic herbivores with a DNA barcoding approach.

Original languageEnglish
Pages (from-to)404-408
Number of pages5
JournalMolecular Ecology Resources
Volume11
Issue number2
DOIs
Publication statusPublished - Mar-2011

Keywords

  • Arctic herbivores
  • barnacle goose
  • bryophytes
  • DNA barcoding
  • faeces
  • BARCODES
  • MOSSES
  • GEESE
  • FOOD

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