[C-11]-DPA-713 and [F-18]-DPA-714 as New PET Tracers for TSPO: A Comparison with [C-11]-(R)-PK11195 in a Rat Model of Herpes Encephalitis

Janine Doorduin*, Hans C. Klein, Rudi A. Dierckx, Michelle James, Michael Kassiou, Erik F. J. de Vries

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

92 Citations (Scopus)

Abstract

Background: Activation of microglia cells plays an important role in neurological diseases. Positron emission tomography (PET) with [C-11]-(R)-PK11195 has already been used to visualize activated microglia cells in neurological diseases. However, [C-11]-(R)-PK11195 may not possess the required sensitivity to visualize mild neuroinflammation. In this study, we evaluated the PET tracers [C-11]-DPA-713 and [F-18]-DPA-714 as agents for imaging of activated microglia in a rat model of herpes encephalitis.

Materials and Methods: Rats were intranasally inoculated with HSV-1. On day 6 or 7 after inoculation, small animal PET studies were performed to compare [C-11]-(R)-PK11195, [C-11]-DPA-713, and [F-18]-DPA-714.

Results: Uptake of [C-11]-DPA-713 in infected brain areas was comparable to that of [C-11]-(R)-PK11195, but [C-11]-DPA-713 showed lower non-specific binding. Non-specific uptake of [F-18]-DPA-714 was lower than that of [C-11]-(R)-PK11195. In the infected brain, total [F-18]-DPA-714 uptake was lower than that of [C-11]-(R)-PK11195, with comparable specific uptake.

Conclusions: [C-11]-DPA-713 may be more suitable for visualizing mild inflammation than [C-11]-(R)-PK11195. In addition, the fact that [F-18]-DPA-714 is an agonist PET tracer opens new possibilities to evaluate different aspects of neuroinflammation. Therefore, both tracers warrant further investigation in animal models and in a clinical setting.

Original languageEnglish
Pages (from-to)386-398
Number of pages13
JournalMolecular Imaging and Biology
Volume11
Issue number6
DOIs
Publication statusPublished - Nov-2009

Keywords

  • Positron emission tomography
  • Neuroinflammation
  • Microglia
  • [C-11]-PK11195
  • TSPO
  • PERIPHERAL BENZODIAZEPINE-RECEPTOR
  • POSITRON-EMISSION-TOMOGRAPHY
  • IN-VIVO
  • MICROGLIAL ACTIVATION
  • MULTIPLE-SCLEROSIS
  • ISCHEMIC-STROKE
  • PARKINSONS-DISEASE
  • SIMPLEX-VIRUS
  • BRAIN
  • BINDING

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