Cascade Reactions in Multicompartmentalized Polymersomes

Ruud J.R.W. Peters, Maïté Marguet, Sébastien Marais, Marco W. Fraaije, Jan C.M. van Hest, Sébastien Lecommandoux

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Enzyme-filled polystyrene-b-poly(3-(isocyano-L-alanyl-aminoethyl)thiophene) (PS-b-PIAT) nanoreactors are encapsulated together with free enzymes and substrates in a larger polybutadiene-b-poly(ethylene oxide) (PB-b-PEO) polymersome, forming a multicompartmentalized structure, which shows structural resemblance to the cell and its organelles. An original cofactor-dependent three-enzyme cascade reaction is performed, using either compatible or incompatible enzymes, which takes place across multiple compartments.
Original languageEnglish
Pages (from-to)146-150
Number of pages5
JournalAngewandte Chemie International Edition
Issue number1
Publication statusPublished - 2014


  • cascade reactions
  • enzyme catalysis
  • nanoreactors
  • polymersomes
  • artificial cells

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