Cationic amphiphiles as delivery system for genes into eukaryotic cells

Volker Oberle, Inge S. Zuhorn, Sandrine Audouy, Udo Bakowsky, Jarmila Smisterová, Jan B.F.N. Engberts, Dick Hoekstra

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Abstract

Cationic liposomes, consisting of synthetic amphiphiles and a so-called helper lipid, rapidly form complexes with DNA, known as lipoplexes. When incubated with cells in culture, the DNA can be delivered into the cell and becomes expressed. Because of these properties, lipoplexes are considered a useful alternative for viral vectors for in vivo genetherapy. Yet, many hurdles have still to be taken. These an illustrated in the present overview, which briefly describes the critical steps involved in overall gene delivery in vitro, ranging from lipoplex formation to nuclear penetration and delivery of the desired gene.

Original languageEnglish
Title of host publicationTARGETING OF DRUGS
EditorsG Gregoriadis, B McCormack
Place of PublicationAMSTERDAM
PublisherI O S PRESS
Pages146-155
Number of pages10
ISBN (Print)1-58603-009-4
Publication statusPublished - 2000
Event10th Meeting of the NATO-Advanced-Studies-Institute on Targeting of Drugs: Strategies for Gene Constructs and Delivery - , Greece
Duration: 24-Jun-19995-Jul-1999

Publication series

NameNATO ADVANCED SCIENCE INSTITUTES SERIES, SERIES A, LIFE SCIENCES
PublisherI O S PRESS
Volume323
ISSN (Print)0258-1213

Other

Other10th Meeting of the NATO-Advanced-Studies-Institute on Targeting of Drugs: Strategies for Gene Constructs and Delivery
Country/TerritoryGreece
Period24/06/199905/07/1999

Keywords

  • ATOMIC-FORCE MICROSCOPY
  • MEMBRANE-FUSION
  • IN-VIVO
  • LIPOSOME COMPLEXES
  • DNA
  • TRANSFECTION
  • EFFICIENT
  • EXPRESSION
  • VESICLES
  • THERAPY

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