Cell-mediated immune responses to influenza vaccination in Wegener's granulomatosis

Albert Holvast*, Aalzen de Haan, Sander van Assen, Coen A. Stegeman, Minke G. Huitema, Anke Huckriede, Cornelis A. Benne, Johanna Westra, Abraham Palache, Jan Wilschut, Cees G. M. Kallenberg, Marc Bijl

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)


Background: Both antibody and cell-mediated immune responses are involved in the defence against influenza. In Wegener's granulomatosis (WG), antibody responses to influenza vaccination appear to be similar to those in healthy controls, but cell-mediated responses have not been studied. Objective: To determine whether cell-mediated responses to influenza vaccination in WG vary from those in controls. Methods: Twenty-five patients with WG and healthy controls received subunit influenza vaccine. Peripheral blood mononuclear cells were obtained before and 1 month after vaccination. Cell-mediated responses to A/H1N1 and A/H3N2 were assessed using interferon γ (IFNγ) ELISpot and intracellular cytokine staining for IFNγ, tumour necrosis factor and interleukin 2. Results: Before vaccination, patients and controls showed similar recall responses to A/H1N1 and A/H3N2. After vaccination, patients and controls showed similar levels of increase in spot-forming cells against A/H1N1 and A/H3N2. By flow cytometry, upon vaccination, proportions of cytokine-producing CD4 T cells increased in patients and controls for A/H1N1 and A/H3N2. Conclusions: Cell-mediated responses to influenza vaccination in patients with WG are comparable to those in healthy controls.
Original languageEnglish
Pages (from-to)924-927
Number of pages4
JournalAnnals of the Rheumatic Diseases
Issue number5
Publication statusPublished - May-2010



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